Cadenas S, Rojas C, Barja G
Department of Animal Biology-II (Animal Physiology), Complutense University, Madrid, Spain.
Pharmacol Toxicol. 1998 Jan;82(1):11-8. doi: 10.1111/j.1600-0773.1998.tb01391.x.
Current information suggests that oxidative damage plays a key role in septic shock induced by endotoxin. This raises the possibility that dietary antioxidant vitamins could protect against endotoxin damage. In this study, the effects of endotoxin administration on protein and lipid oxidative damage and endogenous antioxidants were studied in the liver of guinea pigs previously supplemented with marginal or optimum levels of dietary vitamin C, vitamin E or both. Vitamins C and E inhibited in vitro lipid peroxidation in endotoxin-treated animals. Endotoxin significantly increased oxidative damage to liver proteins in animals receiving low doses of both vitamins, a result described here for the first time. This increase was totally prevented in guinea pigs supplemented with vitamin C alone or in combination with vitamin E, a treatment which strongly increased liver ascorbate. Vitamin C caused small significant increases in superoxide dismutase and glutathione, increased uric acid, and synergically increased alpha-tocopherol levels in vitamin E-supplemented animals treated with endotoxin. The results show that dietary vitamin C protects against endotoxin-induced oxidative damage to proteins in the guinea pig liver. This seems mainly due to a direct protective effect of the increased hepatic ascorbate levels present in vitamin C-supplemented animals.
目前的信息表明，氧化损伤在内毒素诱导的脓毒症休克中起关键作用。这增加了膳食抗氧化维生素可以预防内毒素损伤的可能性。在本研究中，我们在预先补充了边缘或最佳水平膳食维生素C、维生素E或两者的豚鼠肝脏中，研究了给予内毒素对蛋白质和脂质氧化损伤以及内源性抗氧化剂的影响。维生素C和E抑制了内毒素处理动物的体外脂质过氧化。内毒素显著增加了接受低剂量两种维生素的动物肝脏蛋白质的氧化损伤，这一结果在此首次报道。在单独补充维生素C或与维生素E联合补充的豚鼠中，这种增加完全得到预防，这种处理强烈增加了肝脏中的抗坏血酸。维生素C使超氧化物歧化酶和谷胱甘肽有小幅显著增加，尿酸增加，并在内毒素处理的补充维生素E的动物中协同增加α-生育酚水平。结果表明，膳食维生素C可预防豚鼠肝脏中内毒素诱导的蛋白质氧化损伤。这似乎主要是由于补充维生素C的动物肝脏中抗坏血酸水平升高的直接保护作用。
