Huang He, Gong Cheng S, Tsao George T
School of Chemical Engineering and Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47906, USA.
Appl Biochem Biotechnol. 2002 Spring;98-100:687-98.
1,3-Propanediol (1,3-PD) has numerous applications from polymers to cosmetics, foods, lubricants, and medicines. Recently, there are strong industrial interests in a new kind of polyester, polytrimethylene terephthalate, with 1,3-PD as a monomer. This new polyester shows significant promise for use in carpeting and textiles. In this article we introduce a mild aerobic fermentation process using a strain screened from Klebsiella pneumoniae ATCC 25955, which is insensitive to oxygen, to produce 1,3-PD. We also describe a two-step fermentation process starting with glucose that was converted into glycerol with a glycerol-producing yeast, followed by K. pneumoniae that converts glycerol into 1,3-PD without intermediate isolation and purification of glycerol.
1,3-丙二醇（1,3-PD）在从聚合物到化妆品、食品、润滑剂和药品等众多领域都有应用。最近，以1,3-PD作为单体的新型聚酯聚对苯二甲酸丙二醇酯引起了强烈的工业兴趣。这种新型聚酯在地毯和纺织品应用方面显示出巨大的潜力。在本文中，我们介绍了一种温和的需氧发酵工艺，该工艺使用从肺炎克雷伯菌ATCC 25955筛选出的对氧气不敏感的菌株来生产1,3-PD。我们还描述了一种两步发酵工艺，该工艺从葡萄糖开始，先用产甘油酵母将其转化为甘油，然后由肺炎克雷伯菌将甘油转化为1,3-PD，无需对甘油进行中间分离和纯化。
