Harris W G
Immunology. 1975 Nov;29(5):835-44.
A total of thirty-five antigen fractions were prepared from adult Schistosoma mansoni by extraction into borate-buffered saline, precipitation at pH 4-6, gel-filtration on Sephadex G-100 and G-200, and ion-exchange chromatography on CM- and DEAE-cellulose. The allergic activity of these antigens was assayed by a modified Prausnitz-Küstner (P-K) type reaction in rats. The results showed that the allergen-reagin axis in rat schistosomiasis is a multicomponent system involving molecules of widely different chemical nature and molecular weight, rather than a simple, single antigen-antibody interaction. The significance of these findings to the use of purified antigens for the diagnosis of schistosomiasis in the field is discussed.
通过用硼酸盐缓冲盐水提取、在pH 4 - 6下沉淀、在葡聚糖凝胶G - 100和G - 200上进行凝胶过滤以及在CM - 和DEAE - 纤维素上进行离子交换色谱法,从成年曼氏血吸虫中总共制备了35种抗原组分。通过在大鼠中进行改良的普劳斯尼茨 - 屈斯特纳(P - K)型反应来测定这些抗原的过敏活性。结果表明,大鼠血吸虫病中的变应原 - 反应素轴是一个多组分系统,涉及化学性质和分子量差异很大的分子,而不是简单的单一抗原 - 抗体相互作用。讨论了这些发现对于在现场使用纯化抗原诊断血吸虫病的意义。