肺炎克雷伯菌中与洗必泰抗性相关的阳离子外流泵基因的克隆
Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae.
作者信息
Fang Chi-Tai, Chen Haur-Chuan, Chuang Yi-Ping, Chang Shan-Chwen, Wang Jin-Town
机构信息
Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
出版信息
Antimicrob Agents Chemother. 2002 Jun;46(6):2024-8. doi: 10.1128/AAC.46.6.2024-2028.2002.
Abstract
Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.
摘要
构建了一株耐洗必泰肺炎克雷伯菌的表达文库，并将其转化到大肠杆菌XLOLR中。经过筛选获得了20个耐洗必泰的转化子。所有克隆都含有一个新的903个核苷酸的基因座。其序列与一种阳离子外流泵相符，因此该基因座被命名为cepA。使用含cepA的质粒进行再次转化，使XLOLR和一株对洗必泰敏感的肺炎克雷伯菌菌株都获得了洗必泰抗性。因此，CepA与洗必泰抗性相关，可能作为一种阳离子外流泵发挥作用。
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