Ramanujam P, Tan W S, Nathan S, Yusoff K
Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, University Putra Malaysia, Selangor, Malaysia.
Arch Virol. 2002 May;147(5):981-93. doi: 10.1007/s00705-001-0778-y.
A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.
将展示在丝状噬菌体M13上的二硫键约束随机七肽文库用于筛选与新城疫病毒（NDV）相互作用的特异性配体。从淘选过程中筛选出了携带氨基酸序列TLTTKLY的融合噬菌体。抗体竞争试验表明，所选噬菌体能够与针对NDV产生的多克隆抗体竞争病毒上的结合位点。通过溶液中的平衡结合试验测定该噬菌体与NDV的结合亲和力，结果显示有两个不同的解离常数，这表明噬菌体在NDV上可能有两个不同的结合位点。序列为CTLTTKLYC的合成肽，无论是线性还是环状构象，均能抑制携带相同序列的噬菌体与NDV的结合。这些肽还能抑制病毒的溶血活性及其在鸡胚中的增殖。
