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与樱桃坏死斑驳叶病相关的凹病毒的分子特征及欧洲樱桃绿环斑驳病毒分离株的全序列测定

Molecular characterization of foveaviruses associated with the cherry necrotic mottle leaf disease and complete sequencing of an European isolate of Cherry green ring mottle virus.

作者信息

Gentit P, Foissac X, Svanella-Dumas L, Peypelut M, Macquaire G, Candresse T

机构信息

CTIFL, Centre de Lanxade, La Force, France.

出版信息

Arch Virol. 2002 May;147(5):1033-42. doi: 10.1007/s00705-001-0715-0.

DOI:10.1007/s00705-001-0715-0
PMID:12021871
Abstract

Analysis of the P1C124 source (associated with the cherry necrotic mottle leaf (CNML) disease) revealed the presence of two different viral agents. The complete nucleotide sequence of one of these agents, P1A, had an overall nucleotide sequence similarity of 83% with a previously sequenced North American isolate of Cherry green ring mottle virus (CGRMV) and should therefore be regarded as an European isolate of CGRMV. Approximately 2 kb of the 5' end of the genome of the second agent, P1B, were also sequenced and were shown to be 82% homologous with Cherry necrotic rusty mottle virus (CNRMV), another member of the Foveavirus genus. The possible involvement of CGRMV-P1A and of CNRMV-P1B in the etiology of the CNML disease is discussed.

摘要

对P1C124来源(与樱桃坏死斑驳叶病(CNML)相关)的分析揭示了两种不同病毒因子的存在。其中一种因子P1A的完整核苷酸序列与先前测序的北美樱桃绿环斑驳病毒(CGRMV)分离株的总体核苷酸序列相似度为83%,因此应被视为CGRMV的欧洲分离株。第二种因子P1B基因组5'端约2 kb也进行了测序,结果显示与凹陷病毒属的另一个成员樱桃坏死锈斑驳病毒(CNRMV)有82%的同源性。文中讨论了CGRMV - P1A和CNRMV - P1B可能在CNML病病因学中的作用。

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