Phytopathology. 2013 Dec;103(12):1287-95. doi: 10.1094/PHYTO-05-13-0140-R.
Virus RNA sequences related to those of the family Betaflexiviridae were amplified from trees affected with the following diseases: cherry twisted leaf, apricot ring pox, cherry necrotic rusty mottle, cherry rusty mottle, and cherry green ring mottle. Phylogenetic analysis of virus sequences obtained from these diseased trees from western North America, along with published sequences of Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV), revealed four major clades. Segregation into these four populations correlated with distinct symptom expression on woody indicators, suggesting that each clade represents a distinct virus species within the family Betaflexiviridae. The viruses occupying each clade were designated clade I: Cherry twisted leaf associated virus, clade II: CNRMV, clade III: Cherry rusty mottle associated virus, and clade IV: CGRMV. Potential recombination events were predicted to occur within and between these viruses, the latter being strongly supported by incongruent phylogenies. Examination of frequency distribution data derived from pairwise sequence comparisons of coat protein coding sequences resulted in a proposal for alternative guidelines for species demarcation for this family of viruses.
从患有以下疾病的树木中扩增出与 Betaflexiviridae 家族相关的病毒 RNA 序列：樱桃扭叶病、李属环斑病、樱桃坏死锈斑病、樱桃锈斑病和樱桃绿环斑驳病。对来自北美西部这些患病树木的病毒序列以及已发表的樱桃绿环斑驳病毒（CGRMV）和樱桃坏死锈斑病毒（CNRMV）序列进行的系统发育分析表明，存在四个主要分支。这些分支的分离与木质指示物上明显的症状表达相关，表明每个分支代表 Betaflexiviridae 家族中的一个独特病毒种。占据每个分支的病毒分别被指定为：第一分支：樱桃扭叶病相关病毒，第二分支：CNRMV，第三分支：樱桃锈斑病相关病毒，第四分支：CGRMV。预测这些病毒内部和之间会发生潜在的重组事件，后者被不一致的系统发育强烈支持。对来自衣壳蛋白编码序列的成对序列比较得出的频率分布数据进行检查，导致对该病毒家族的种划分提出了替代准则。
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