Falsini Benedetto, Riva Charles E, Logean Eric
Istituto di Oftalmologia, Università Cattolica del S. Cuore, Rome, Italy.
Klin Monbl Augenheilkd. 2002 Apr;219(4):296-8. doi: 10.1055/s-2002-30657.
The relationship between the flicker-evoked changes in optic nerve blood flow (F onh ) and neural retinal activity was investigated by laser Doppler flowmetry and electroretinogram (ERG), respectively.
In five normal subjects F onh was continuously recorded before, during and after exposure to green flicker modulation (30 degrees field at the posterior pole), at different levels of mean illuminance between 0.9 and 13.5 lux. During flicker stimulation, ERGs were simultaneously recorded with F onh. The flicker-evoked changes in F onh ( RF onh ) and the amplitudes of the first (1F) and second (2F) harmonic component of the ERG were measured.
By increasing mean flicker illuminance, RF onh and ERG 2F, but not 1F amplitude first increased and then saturated beyond 10 lux. RF onh and the corresponding 2F amplitudes, recorded at the various mean illuminances, showed a significant positive correlation (r = 0.79, p < 0.01).
Under specific experimental conditions, changes in human RF onh are quantitatively correlated with those of the flicker ERG 2F amplitudes. Since the 2F component reflects, unlike 1F, a strong contribution from inner retina, the present findings support the presence of an association between vasoactivity and inner retinal activity changes in the human eye. This finding may provide a new approach to the study of neuro-ophthalmic disorders.
分别通过激光多普勒血流仪和视网膜电图（ERG）研究了闪烁诱发的视神经血流变化（F onh）与神经视网膜活动之间的关系。
在5名正常受试者中，于0.9至13.5勒克斯之间的不同平均照度水平下，在暴露于绿色闪烁调制（后极30度视野）之前、期间和之后连续记录F onh。在闪烁刺激期间，与F onh同时记录ERG。测量闪烁诱发的F onh变化（RF onh）以及ERG的第一（1F）和第二（2F）谐波分量的振幅。
通过增加平均闪烁照度，RF onh和ERG 2F，但不是1F振幅首先增加，然后在超过10勒克斯时达到饱和。在各种平均照度下记录的RF onh和相应的2F振幅显示出显著的正相关（r = 0.79，p < 0.01）。
在特定实验条件下，人类RF onh的变化与闪烁ERG 2F振幅的变化在数量上相关。由于2F分量与1F不同，它反映了来自视网膜内层的强烈贡献，因此本研究结果支持人眼中血管活性与视网膜内层活性变化之间存在关联。这一发现可能为神经眼科疾病的研究提供一种新方法。
