Aviñó J, Díaz-Llopis M, España E, Johnsen-Soriano S, Romero B, Marín N, Muriach M, Bosch-Morell F, Romero F J
Servicio de Oftalmología, Hospital Universitario La Fe, Valencia, España.
Arch Soc Esp Oftalmol. 2002 May;77(5):263-8.
To assess the involvement of oxidative stress in optic nerves after chronic intake of ethanol in adult rats, when compared to animals fed with an ethanol-free isocaloric diet.
Male Sprague-Dawley rats were used throughout the study. They were fed an ethanol-containing liquid diet, whereas the pair-fed group was given an ethanol-free isocaloric diet. After six weeks of the experiment, optic nerves were extracted and markers of oxidative stress were assayed, i.e., antioxidants such as glutathione and lipid peroxidation products such as malondialdehyde (MDA).
The GSH content in the optic nerves of ethanol-fed animals was significantly reduced, and the concentration of MDA was significantly higher in this group when compared with the pair-fed group. Time-course of body weight of animals in both groups varied identically throughout the six weeks of the experiment.
The increase in lipid peroxidation products (MDA), together with the decrease in cellular antioxidants (GSH) confirm, in this experimental model, the involvement of oxidative stress in ethanol-induced toxicity of the optic nerves of rats. In view of the body weight time course, the influence of nutritional status on the parameters studied could also be discarded (Arch Soc Esp Oftalmol 2002; 77: 263-268).
与喂食无乙醇等热量饮食的动物相比，评估成年大鼠长期摄入乙醇后氧化应激对视神经的影响。
在整个研究过程中使用雄性Sprague-Dawley大鼠。给它们喂食含乙醇的液体饮食，而配对喂食组给予无乙醇等热量饮食。实验六周后，提取视神经并检测氧化应激标志物，即抗氧化剂如谷胱甘肽和脂质过氧化产物如丙二醛（MDA）。
与配对喂食组相比，喂食乙醇的动物视神经中谷胱甘肽含量显著降低，且该组丙二醛浓度显著更高。在整个六周的实验过程中，两组动物体重的时间进程变化相同。
在该实验模型中，脂质过氧化产物（MDA）增加以及细胞抗氧化剂（GSH）减少证实了氧化应激参与乙醇诱导的大鼠视神经毒性。鉴于体重的时间进程，营养状况对所研究参数的影响也可排除（《西班牙眼科协会档案》2002年；77：263 - 268）。
