Johnsen-Soriano S, Genovés J M, Romero B, García-Delpech S, Muriach M, Sancho-Tello M, Bosch-Morell F, Romero F J
Fundación Oftalmológica del Mediterráneo, Valencia, España.
Arch Soc Esp Oftalmol. 2007 Dec;82(12):757-62. doi: 10.4321/s0365-66912007001200008.
To assess the involvement of biochemical and functional changes to the retina after chronic ethanol intake in adult rats, and the capacity of the antioxidant ebselen to prevent these changes.
Male Sprague-Dawley rats were used in the study. They were fed an ethanol-containing liquid diet, whereas a control group was given an ethanol-free isocaloric diet. After six weeks of experiment, the eyes were extracted and homogenized without the lens, and markers of oxidative stress were assayed, i.e., glutathione (GSH) and malondialdehyde (MDA) as an intracellular antioxidant and a lipid peroxidation product, respectively. Moreover, retinal function was assessed by electroretinogram (ERG).
The retinal MDA concentration was significantly increased in the ethanol-fed animals compared to controls, whereas the GSH content was significantly reduced in the ethanol-fed group compared to controls. Ethanol also induced a decrease in ERG b-wave amplitude. Ebselen treatment restored the MDA and GSH concentrations and ERG b-wave amplitude to control values.
These results indicate that chronic alcohol consumption alone and without the influence of nutritional factors alters the retinal redox status as well as its function (ERG). Further studies are required to better understand the protective mechanism of ebselen in this experimental model of chronic alcoholism.
评估成年大鼠长期摄入乙醇后视网膜的生化和功能变化，以及抗氧化剂依布硒啉预防这些变化的能力。
本研究使用雄性斯普拉格-道利大鼠。给它们喂食含乙醇的液体饲料，而对照组则给予无乙醇的等热量饲料。实验六周后，取出眼睛并去除晶状体后进行匀浆，检测氧化应激标志物，即分别作为细胞内抗氧化剂和脂质过氧化产物的谷胱甘肽（GSH）和丙二醛（MDA）。此外，通过视网膜电图（ERG）评估视网膜功能。
与对照组相比，喂食乙醇的动物视网膜MDA浓度显著升高，而喂食乙醇组的GSH含量与对照组相比显著降低。乙醇还导致ERG b波振幅降低。依布硒啉治疗使MDA和GSH浓度以及ERG b波振幅恢复到对照值。
这些结果表明，在没有营养因素影响的情况下，长期单独饮酒会改变视网膜的氧化还原状态及其功能（ERG）。需要进一步研究以更好地了解依布硒啉在这种慢性酒精中毒实验模型中的保护机制。
