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[肌浆网囊泡存在下平面双层脂质膜的通透性]

[Permeability of plane bilayer lipid membranes in the presence of sarcoplasmic reticulum vesicles].

作者信息

Sergeeva N S, Poglazov A F, Vladimirov Iu A

出版信息

Biofizika. 1975 Nov-Dec;20(6):1029-32.

PMID:1203294
Abstract

Interaction between vesicles of sarcoplasmic reticulum (VSR) and bilayer lipid membranes (BLM) was investigated. VSR were added into the membrane-surrounding solution. For the formation of complex VSR-BLM the surface of BLM was charged positively by adding 10(-6) M acetyltrimethylammonium bromide and the transmembrane electrical potential was applied in the negative direction (the positive direction was chosen from inner section of camera with VSR to outer section). The formation of complex VSR-BLM proceeds via two stages. The second stage is accompanied by the formation of nonselective channels of conductivity, perhaps, aqueous pores.

摘要

研究了肌浆网小泡(VSR)与双层脂质膜(BLM)之间的相互作用。将VSR添加到膜周围溶液中。为了形成VSR - BLM复合物,通过添加10⁻⁶ M溴化乙酰三甲基铵使BLM表面带正电,并向负方向施加跨膜电势(正方向是从含有VSR的相机内部区域指向外部区域)。VSR - BLM复合物的形成过程分两个阶段进行。第二阶段伴随着电导率非选择性通道的形成,可能是水孔。

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引用本文的文献

1
Monovalent ion and calcium ion fluxes in sarcoplasmic reticulum.肌浆网中的单价离子和钙离子通量
Mol Cell Biochem. 1983;55(1):65-82. doi: 10.1007/BF00229243.
2
Voltage-gated cation conductance channel from fragmented sarcoplasmic reticulum: steady-state electrical properties.来自破碎肌浆网的电压门控阳离子电导通道:稳态电学特性
J Membr Biol. 1978 Apr 20;40(1):1-23. doi: 10.1007/BF01909736.