Benesch Johan, Tengvall Pentti
The Department of Physics and Measurement Technology, Biology and Chemistry Linköpings Universitet, Sweden.
Biomaterials. 2002 Jun;23(12):2561-8. doi: 10.1016/s0142-9612(01)00391-x.
Chitosan was recently indicated to enhance osteogenesis, improve wound healing but to activate the coagulation and the complement systems. In the present study approximately 10 nm thick chitosan film were prepared on aminopropyltriethoxysilane (APTES) coated silicon. The surfaces were incubated in serum or plasma and subsequently in antibodies towards key complement and contact activation of coagulation proteins. The deposited amounts were compared with those on hydrophilic and hydrophobic silicon, APTES and IgG coated reference samples. Although large amounts of serum deposited to chitosan only a weak transient activation of the complement system and no activation of the intrinsic pathway was observed. Upon acetylation the chitosan layer became a strong activator of the alternative pathway of the complement. After incubation in human plasma anti-fibrinogen deposited onto chitosan but not onto the acetylated chitosan, a finding that may explain previous observations of procoagulant activity by chitosan.
壳聚糖最近被指出可促进成骨作用、改善伤口愈合，但会激活凝血和补体系统。在本研究中，在氨基丙基三乙氧基硅烷（APTES）涂层的硅片上制备了厚度约为10纳米的壳聚糖薄膜。将这些表面在血清或血浆中孵育，随后与针对关键补体和凝血蛋白接触激活的抗体孵育。将沉积量与在亲水性和疏水性硅片、APTES和IgG涂层参考样品上的沉积量进行比较。尽管大量血清沉积到壳聚糖上，但仅观察到补体系统的微弱瞬时激活，且未观察到内源性途径的激活。乙酰化后，壳聚糖层成为补体替代途径的强激活剂。在人血浆中孵育后，抗纤维蛋白原沉积在壳聚糖上，而不是乙酰化壳聚糖上，这一发现可能解释了壳聚糖先前促凝血活性的观察结果。
