College of Life Sciences, Key Laboratory of Bioactive Materials (Ministry of Education),Tianjin Center Hospital of Obstetrics and Gynecology, State Key Laboratory of Medicine Chemical Biology, Nankai University, Tianjin, China.
Department of Orthopedics, The Second Hospital of Tianjin Medical University, Tianjin, China.
Nat Commun. 2021 Aug 5;12(1):4733. doi: 10.1038/s41467-021-24972-2.
Developing an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.
开发一种能够在民用和战场环境下引导原位组织再生的抗感染形状记忆止血海绵，以治疗不可压缩性出血，这仍然是一个挑战。在这里，我们通过结合 3D 打印微纤维溶出、冷冻干燥和表面活性修饰，设计了具有高度互连微通道的止血壳聚糖海绵。我们证明了具有微通道的烷基化壳聚糖海绵（MACS）具有吸水和吸血的能力，以及快速的形状恢复能力。我们发现，与临床使用的纱布、明胶海绵、CELOX™和 CELOX™-纱布相比，MACS 在致死性正常和肝素化大鼠和猪肝穿孔伤口模型中具有更高的促凝和止血能力。我们证明了它对金黄色葡萄球菌和大肠杆菌的抗感染活性，以及在大鼠肝缺损模型中促进实质细胞浸润、血管生成和组织整合的能力。总的来说，MACS 在治疗致命性不可压缩性出血和促进伤口愈合方面具有有前景的临床转化潜力。
