通过实时聚合酶链反应对呼吸道肺炎衣原体感染进行定量检测。
Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR.
作者信息
Kuoppa Yvonne, Boman Jens, Scott Lena, Kumlin Urban, Eriksson Iréne, Allard Annika
机构信息
Department of Virology, University of Umeå, Umeå, Sweden.
出版信息
J Clin Microbiol. 2002 Jun;40(6):2273-4. doi: 10.1128/JCM.40.6.2273-2274.2002.
Abstract
Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.
摘要
使用不同的呼吸道样本对实时荧光定量聚合酶链反应（Real-time PCR）作为肺炎衣原体感染的定量诊断方法进行了评估。实时荧光定量聚合酶链反应的效率等同于或优于巢式降落聚合酶链反应。本研究证实痰液是检测正在进行的肺炎衣原体感染的最佳采样材料。
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本文引用的文献
1
Quantification of human cytomegalovirus DNA by real-time PCR.
J Clin Microbiol. 2001 Feb;39(2):772-5. doi: 10.1128/JCM.39.2.772-775.2001.
2
Simultaneous detection of influenza viruses A and B using real-time quantitative PCR.
J Clin Microbiol. 2001 Jan;39(1):196-200. doi: 10.1128/JCM.39.1.196-200.2001.
3
Molecular diagnosis of Chlamydia pneumoniae infection.
J Clin Microbiol. 1999 Dec;37(12):3791-9. doi: 10.1128/JCM.37.12.3791-3799.1999.
4
Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis.
J Clin Microbiol. 1998 Jul;36(7):1964-8. doi: 10.1128/JCM.36.7.1964-1968.1998.
5
Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA.
J Infect Dis. 1997 Jun;175(6):1523-6. doi: 10.1086/516492.
6
Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.
Clin Microbiol Rev. 1997 Apr;10(2):242-56. doi: 10.1128/CMR.10.2.242.
7
Chlamydia pneumoniae (TWAR).
Clin Microbiol Rev. 1995 Oct;8(4):451-61. doi: 10.1128/CMR.8.4.451.
8
Detection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR.
J Clin Pathol. 1993 Apr;46(4):313-7. doi: 10.1136/jcp.46.4.313.
9
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
PCR Methods Appl. 1995 Jun;4(6):357-62. doi: 10.1101/gr.4.6.357.
10
A new respiratory tract pathogen: Chlamydia pneumoniae strain TWAR.
J Infect Dis. 1990 Apr;161(4):618-25. doi: 10.1093/infdis/161.4.618.
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