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本文引用的文献

1
Detection of human cytomegalovirus DNA by real-time quantitative PCR.通过实时定量聚合酶链反应检测人巨细胞病毒DNA
J Clin Microbiol. 2000 Jul;38(7):2734-7. doi: 10.1128/JCM.38.7.2734-2737.2000.
2
Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation.用于骨髓移植后巨细胞病毒感染早期诊断和监测的实时自动化聚合酶链反应
J Clin Microbiol. 2000 Jul;38(7):2536-42. doi: 10.1128/JCM.38.7.2536-2542.2000.
3
Real-time monitoring of cytomegalovirus infections after stem cell transplantation using the TaqMan polymerase chain reaction assays.使用TaqMan聚合酶链反应分析法对干细胞移植后巨细胞病毒感染进行实时监测。
Transplantation. 2000 Apr 27;69(8):1733-6. doi: 10.1097/00007890-200004270-00037.
4
Multiplex polymerase chain reaction for the evaluation of cytomegalovirus DNA load in organ transplant recipients.用于评估器官移植受者巨细胞病毒DNA载量的多重聚合酶链反应
J Med Virol. 2000 Jun;61(2):251-8.
5
Quantitative analysis of cytomegalovirus load using a real-time PCR assay.使用实时聚合酶链反应测定法对巨细胞病毒载量进行定量分析。
J Med Virol. 2000 Apr;60(4):455-62. doi: 10.1002/(sici)1096-9071(200004)60:4<455::aid-jmv14>3.0.co;2-q.
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Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA.比较不同实时PCR方法用于人巨细胞病毒DNA的定量检测
Clin Chem. 1999 Nov;45(11):1932-7.
7
TaqMan PCR-based gene dosage assay for predictive testing in individuals from a cancer family with INK4 locus haploinsufficiency.基于TaqMan PCR的基因剂量分析，用于对具有INK4基因座单倍体不足的癌症家族个体进行预测性检测。
Clin Chem. 1999 Jul;45(7):982-6.
8
Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients.定量聚合酶链反应预测肾移植受者症状性巨细胞病毒感染的发生并评估其对更昔洛韦治疗的反应
J Infect Dis. 1998 Sep;178(3):626-35. doi: 10.1086/515383.
9
Quantitation of cytomegalovirus: methodologic aspects and clinical applications.巨细胞病毒的定量分析：方法学方面及临床应用
Clin Microbiol Rev. 1998 Jul;11(3):533-54. doi: 10.1128/CMR.11.3.533.
10
Summary of the International Consensus Symposium on Advances in the Diagnosis, Treatment and Prophylaxis and Cytomegalovirus Infection.国际巨细胞病毒感染诊断、治疗、预防进展共识研讨会综述
Antiviral Res. 1996 Nov;32(3):119-40. doi: 10.1016/s0166-3542(96)01006-6.

通过实时聚合酶链反应对人巨细胞病毒DNA进行定量分析。

Quantification of human cytomegalovirus DNA by real-time PCR.

作者信息

Gault E, Michel Y, Dehée A, Belabani C, Nicolas J C, Garbarg-Chenon A

机构信息

Laboratoire de Virologie, Hôpital Armand Trousseau, Paris, France.

出版信息

J Clin Microbiol. 2001 Feb;39(2):772-5. doi: 10.1128/JCM.39.2.772-775.2001.

DOI:10.1128/JCM.39.2.772-775.2001
PMID:11158149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87818/
Abstract

A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).

摘要

开发了一种定量实时聚合酶链反应（PCR）检测方法，用于测量外周血白细胞（PBL）中的人巨细胞病毒（HCMV）DNA载量。通过对一种细胞基因（白蛋白）进行定量，将PBL中的HCMV DNA载量标准化。实时PCR检测结果与HCMV pp65抗原血症检测结果相关（P < 0.0001）。