Nichols David S, Presser Kirsty A, Olley June, Ross Tom, McMeekin Tom A
Centre for Food Safety and Quality, School of Agricultural Science, University of Tasmania, Hobart, Tasmania 7001, Australia.
Appl Environ Microbiol. 2002 Jun;68(6):2809-13. doi: 10.1128/AEM.68.6.2809-2813.2002.
The fatty acid composition of Listeria monocytogenes Scott A was determined by close-interval sampling over the entire biokinetic temperature range. There was a high degree of variation in the percentage of branched-chain fatty acids at any given temperature. The percentage of branched C17 components increased with growth temperature in a linear manner. However, the percentages of iso-C15:0 (i15:0) and anteiso-C15:0 (a15:0) were well described by third-order and second-order polynomial curves, respectively. There were specific temperature regions where the proportion of branched-chain fatty acids deviated significantly from the trend established over the entire growth range. In the region from 12 to 13 degrees C there were significant deviations in the percentages of both i15:0 and a15:0 together with a suggested deviation in a17:0, resulting in a significant change in the total branched-chain fatty acids. In the 31 to 33 degrees C region the percentage of total branched-chain components exhibited a significant deviation. The observed perturbations in fatty acid composition occurred near the estimated boundaries of the normal physiological range for growth.
通过在整个生物动力学温度范围内进行近距离采样，测定了单核细胞增生李斯特菌Scott A的脂肪酸组成。在任何给定温度下，支链脂肪酸的百分比都存在高度变化。支链C17成分的百分比随生长温度呈线性增加。然而，异-C15:0（i15:0）和anteiso-C15:0（a15:0）的百分比分别由三阶和二阶多项式曲线很好地描述。存在特定的温度区域，其中支链脂肪酸的比例明显偏离在整个生长范围内建立的趋势。在12至13摄氏度的区域中，i15:0和a15:0的百分比均存在显著偏差，同时a17:0也有偏差迹象，导致总支链脂肪酸发生显著变化。在31至33摄氏度的区域中，总支链成分的百分比表现出显著偏差。观察到的脂肪酸组成扰动发生在生长正常生理范围的估计边界附近。
