Nakashima M, Mizunuma K, Murakami T, Akamine A
Department of Clinical Oral Molecular Biology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Gene Ther. 2002 Jun;9(12):814-8. doi: 10.1038/sj.gt.3301692.
The long-term goal of dental treatment is to preserve teeth and prolong their function. In dental caries an efficient method is to cap the exposed dental pulp and conserve the pulp tissue with reparative dentin. We examined whether growth/differentiation factor 11 (GDF11), a morphogen could enhance the healing potential of pulp tissue to induce differentiation of pulp stem cells into odontoblasts by electroporation-mediated gene delivery. Recombinant human GDF11 induced the expression of dentin sialoprotein (Dsp), a differentiation marker for odontoblasts, in mouse dental papilla mesenchyme in organ culture. The Gdf11 cDNA plasmid which was transferred into mesenchymal cells derived from mouse dental papilla by electroporation, induced the expression of Dsp. The in vivo transfer of Gdf11 by electroporation stimulated the reparative dentin formation during pulpal wound healing in canine teeth. These results provide the scientific basis and rationale for gene therapy for endodontic treatments in oral medicine and dentistry.
牙科治疗的长期目标是保留牙齿并延长其功能。在龋齿治疗中，一种有效的方法是覆盖暴露的牙髓并用修复性牙本质保存牙髓组织。我们研究了形态发生素生长/分化因子11（GDF11）是否可以通过电穿孔介导的基因传递增强牙髓组织的愈合潜力，从而诱导牙髓干细胞分化为成牙本质细胞。重组人GDF11在器官培养的小鼠牙乳头间充质中诱导了成牙本质细胞分化标志物牙本质涎蛋白（Dsp）的表达。通过电穿孔转入源自小鼠牙乳头的间充质细胞的Gdf11 cDNA质粒诱导了Dsp的表达。电穿孔介导的Gdf11体内转移刺激了犬牙髓损伤愈合过程中修复性牙本质的形成。这些结果为口腔医学和牙科学中牙髓病治疗的基因治疗提供了科学依据和理论基础。
