Zusterzeel P L M, Steegers-Theunissen R P M, Harren F J M, Stekkinger E, Kateman H, Timmerman B H, Berkelmans R, Nieuwenhuizen A, Peters W H M, Raijmakers M T M, Steegers E A P
Department of Obstetrics and Gynaecology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Hypertens Pregnancy. 2002;21(1):39-49. doi: 10.1081/PRG-120002908.
An increase in reactive oxygen species (ROS) and lipid peroxides and a comprised antioxidant status has been implicated in the pathophysiology of severe preeclampsia. This study investigates whether oxidative stress and impaired antioxidant systems also contribute to milder forms of hypertensive disorders in pregnancy. Furthermore, ethene in exhaled air, a noninvasive measure for oxidative stress, was evaluated and compared with two other more established biomarkers.
Ethene in exhaled air, plasma protein carbonyls, and the ratio of free glutathione/oxidized glutathione (GSHfree/GSHox) as markers for oxidative stress as well as the antioxidants vitamins C and E, uric acid, glutathione, and the oxygen radical absorbance capacity (ORAC) in plasma were measured in 30 healthy nonpregnant, 14 normal pregnant, 9 women with pregnancy-induced hypertension (PIH), and 14 preeclamptic women. Pregnant participants were measured during pregnancy and after delivery.
Women suffering from PIH and preeclampsia showed higher levels of the antioxidants vitamin E and uric acid, and lower levels of vitamin C compared with normal pregnant and nonpregnant women. All markers for oxidative stress were comparable between groups. Ethene levels showed a positive correlation with protein carbonyls but no correlation could be demonstrated with the free glutathione/oxidised glutathione ratio.
PIH and preeclampsia are associated with minor alterations in antioxidant levels without signs of oxidative stress. Detection of ethene in exhaled air seems a promising noninvasive method to study lipid peroxidation but further research in more severe preeclampsia is needed.
活性氧（ROS）和脂质过氧化物增加以及抗氧化状态受损与重度子痫前期的病理生理学有关。本研究调查氧化应激和抗氧化系统受损是否也导致妊娠期较轻形式的高血压疾病。此外，对呼出气体中的乙烯（一种氧化应激的非侵入性测量指标）进行了评估，并与另外两种更成熟的生物标志物进行了比较。
测量了30名健康未孕女性、14名正常妊娠女性、9名妊娠高血压（PIH）女性和14名子痫前期女性呼出气体中的乙烯、血浆蛋白羰基以及作为氧化应激标志物的游离谷胱甘肽/氧化型谷胱甘肽比值（GSHfree/GSHox），以及血浆中的抗氧化剂维生素C和E、尿酸、谷胱甘肽和氧自由基吸收能力（ORAC）。对妊娠女性在孕期和产后进行了测量。
与正常妊娠和未孕女性相比，患有PIH和子痫前期的女性抗氧化剂维生素E和尿酸水平较高，而维生素C水平较低。各组之间所有氧化应激标志物均具有可比性。乙烯水平与蛋白羰基呈正相关，但与游离谷胱甘肽/氧化型谷胱甘肽比值无相关性。
PIH和子痫前期与抗氧化剂水平的轻微改变有关，无氧化应激迹象。检测呼出气体中的乙烯似乎是一种有前景的研究脂质过氧化的非侵入性方法，但需要对更严重的子痫前期进行进一步研究。
