一种来自冲绳海葵（学名：Phyllodiscus semoni，日语名“unbachi-isoginchaku”）刺丝囊毒液的新型多肽毒素。

A new polypeptide toxin from the nematocyst venom of an Okinawan sea anemone Phyllodiscus semoni (Japanese name "unbachi-isoginchaku").

作者信息

Nagai Hiroshi, Oshiro Naomasa, Takuwa-Kuroda Kyoko, Iwanaga Setsuko, Nozaki Masatoshi, Nakajima Terumi

机构信息

Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto, Mishima, Osaka 618-8503, Japan.

出版信息

Biosci Biotechnol Biochem. 2002 Dec;66(12):2621-5. doi: 10.1271/bbb.66.2621.

DOI:10.1271/bbb.66.2621

PMID:12596857

Abstract

The venomous sea anemone Phyllodiscus semoni causes cases of severe stinging. We isolated Phyllodiscus semoni toxin 20A (PsTX-20A), a hemolytic and lethal polypeptide (20 kDa), from the nematocyst venom of this species for the first time. Furthermore, we sequenced the cDNA encoding PsTX-20A. The deduced amino acid sequence of PsTX-20A showed that this toxin was a new member of the actinoporin family, which consists of several cytolytic polypeptides originating from sea anemones. PsTX-20A showed lethal toxicity to the shrimp Palaemon paucidens when administered via intraperitoneal injection (LD50, 50 microg/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED50, 80 ng/ml).

摘要

有毒的海葵半环真叶螅会导致严重的蜇伤病例。我们首次从该物种的刺丝囊毒液中分离出了半环真叶螅毒素20A（PsTX - 20A），这是一种具有溶血和致死作用的多肽（20 kDa）。此外，我们对编码PsTX - 20A的cDNA进行了测序。PsTX - 20A推导的氨基酸序列表明，该毒素是孔蛋白家族的一个新成员，该家族由几种源自海葵的细胞溶解多肽组成。通过腹腔注射给予PsTX - 20A时，对日本对虾表现出致死毒性（LD50，50微克/千克），对0.8%的绵羊红细胞具有溶血活性（ED50，80纳克/毫升）。