Bae H S, Yamagishi T, Suwa Y
Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Appl Microbiol Biotechnol. 2002 Jun;59(1):118-24. doi: 10.1007/s00253-002-0974-z. Epub 2002 Apr 16.
Microbial consortia capable of degrading 3-chlorophenol (3-CP) were enriched in continuous up-flow column reactors under circum-denitrifying conditions. 3-CP degradation capability was developed and sustained when 3-CP was supplied at 16-21 microM, although suppression of the 3-CP degradation capability was observed when 3-CP was supplied at 42 microM. When 3-CP was stably degraded, the ratio of nitrate consumption to 3-CP degradation approached the theoretical stoichiometric value, which was calculated by assuming a 3-CP degradation-dependent nitrate reduction. Batch-incubation experiments demonstrated that the microbial consortium that was enriched in the column reactors required either nitrate or oxygen for degrading 3-CP, while 3-CP was not degraded under sulfate-degrading conditions. Although many attempts were made to sustain the microbial 3-CP degradation capability under denitrifying conditions, mostly in batch cultures, none of them have been successful so far. Therefore, the results obtained in this study may be the first to demonstrate sustainable 3-CP degradation capability under circum-denitrifying conditions.
在循环反硝化条件下，通过连续上流式柱式反应器富集了能够降解3-氯苯酚（3-CP）的微生物群落。当3-CP的供应浓度为16-21微摩尔时，3-CP降解能力得以发展并维持，不过当3-CP供应浓度为42微摩尔时，观察到3-CP降解能力受到抑制。当3-CP稳定降解时，硝酸盐消耗量与3-CP降解量的比值接近理论化学计量值，该值是通过假设3-CP降解依赖的硝酸盐还原计算得出的。批次培养实验表明，在柱式反应器中富集的微生物群落降解3-CP需要硝酸盐或氧气，而在硫酸盐还原条件下3-CP不会降解。尽管人们进行了许多尝试来维持反硝化条件下微生物的3-CP降解能力，大多是在批次培养中进行的，但迄今为止均未成功。因此，本研究获得的结果可能首次证明了在循环反硝化条件下可持续的3-CP降解能力。
