Schleicher A, Zilles K, Wingert F, Kretschmann H J
Microsc Acta. 1975 Nov;77(4):316-30.
A method has been described for correction of a bias, when cells with more than one nucleolus are counted by counting their nucleoli. The true numbers of cells and nucleoli per cell can be estimated by a system of linear equations, with coefficients depending on the thickness of the histological specimen, the average nuclear diameter and the probabilities of nuclear slices, which one gets by cutting a cell nucleus with a certain number of nucleoli. The presuppositions for this method have been discussed with respect to different practical situations. Uncorrected and corrected numbers of motoneurons in the nucl. n. oculomotorii of Tupaia belangeri have been compared.
本文描述了一种校正偏差的方法，该偏差出现在通过计数具有多个核仁的细胞的核仁来对细胞进行计数时。每个细胞的真实细胞数和核仁数可以通过一个线性方程组来估计，方程组的系数取决于组织学标本的厚度、平均核直径以及通过切割具有一定数量核仁的细胞核所获得的核切片概率。针对不同的实际情况，已对该方法的前提条件进行了讨论。比较了树鼩动眼神经核中未校正和校正后的运动神经元数量。
