Zilles K, Schleicher A, Kretschmann H J
Anat Embryol (Berl). 1976 Apr 21;149(1):15-28. doi: 10.1007/BF00315082.
The development during ontogenesis of the nuclear and nucleolar diameters and of the numbers of nucleoli per nucleus in the nucl. n. oculomotorii of 32 male Tupaia belangeri are described. The measurements of the nucleolar diameters were done automatically using the Micro-Videomat (Zeiss, Oberkochen). Increase of the nuclear and nucleolar diameters yielded a nearly sigmoid-shaped curve, which was fitted using the logistic growth function. The "ideal" value of the nucleolar diameter is 3.7 mum with a half value time of 22 days of ontogenesis and an enlargement factor of 1.53. The respective parameters for the nucleus are 12.2 mum, 28 days and 1.27. The ratio of the nuclear to nucleolar diameters decreases exponentially during ontogenesis to a value of 3.3. The validity of these easily determinable morphologic parameters for the RNA-synthesis is discussed. The number of nuclei with 2 or 3 nucleoli decreases during ontogenesis. The relevance of this fact for cell counting is also discussed.
描述了32只雄性树鼩动眼神经核中核直径、核仁直径及每个细胞核仁数量在个体发育过程中的变化情况。核仁直径的测量使用Micro-Videomat（蔡司，奥伯科亨）自动完成。核直径和核仁直径的增加产生了一条近似S形的曲线，该曲线使用逻辑生长函数进行拟合。核仁直径的“理想”值为3.7μm，个体发育的半衰期为22天，放大因子为1.53。细胞核的相应参数为12.2μm、28天和1.27。核直径与核仁直径的比值在个体发育过程中呈指数下降，降至3.3。讨论了这些易于确定的形态学参数对RNA合成的有效性。具有2个或3个核仁的细胞核数量在个体发育过程中减少。还讨论了这一事实与细胞计数的相关性。
