van Beekvelt Mireille C P, van Engelen Baziel G M, Wevers Ron A, Colier Willy N J M
Neuromuscular Centre Nijmegen, Institute of Neurology, University Medical Centre Nijmegen, The Netherlands.
Clin Physiol Funct Imaging. 2002 May;22(3):210-7. doi: 10.1046/j.1475-097x.2002.00420.x.
The aim of this study was to investigate the performance of in vivo quantitative near-infrared spectroscopy (NIRS) in skeletal muscle at various workloads. NIRS was used for the quantitative measurement of O2 consumption (mVO2) in the human flexor digitorum superficialis muscle at rest and during rhythmic isometric handgrip exercise in a broad range of work intensities (10-90% MVC=maximum voluntary contraction force). Six subjects were tested on three separate days. No significant differences were found in mVO2 measured over different days with the exception of the highest workload. The within-subject variability for each workload measured over the three measurements days ranged from 15.7 to 25.6% and did not increase at the high workloads. The mVO2 was 0.14 +/- 0.01 mlO2 min-1 100 g-1 at rest and increased roughly 19 times to 2.68 +/- 0.58 mlO2 min-1 100 g-1 at 72% MVC. These results show that local muscle oxygen consumption at rest as well as during exercise at a broad range of work intensities can be measured reliably by NIRS, applied to a uniform selected subject population. This is of great importance as direct local measurement of mVO2 during exercise is not possible with the conventional techniques. The method is robust enough to measure over separate days and at various workloads and can therefore contribute to a better understanding of human physiology in both the normal and pathological state of the muscle.
本研究的目的是调查体内定量近红外光谱（NIRS）在不同工作负荷下对骨骼肌的检测性能。NIRS用于定量测量人体浅屈指肌在静息状态以及在广泛工作强度（10 - 90%MVC = 最大自主收缩力）的节律性等长握力运动过程中的氧气消耗量（mVO2）。六名受试者在三个不同日期进行了测试。除了最高工作负荷外，不同日期测量的mVO2没有显著差异。在三个测量日测量的每个工作负荷下的受试者内变异性范围为15.7%至25.6%，在高工作负荷下并未增加。静息时mVO2为0.14±0.01 mlO2 min-1 100 g-1，在72%MVC时大致增加19倍至2.68±0.58 mlO2 min-1 100 g-1。这些结果表明，应用于统一选定的受试者群体时，NIRS能够可靠地测量静息以及广泛工作强度运动期间的局部肌肉氧消耗。这非常重要，因为传统技术无法在运动期间直接进行mVO2的局部测量。该方法足够稳健，可以在不同日期和各种工作负荷下进行测量，因此有助于更好地理解肌肉正常和病理状态下的人体生理学。
