Wellde B T, Diggs C L, Anderson S
Bull World Health Organ. 1979;57 Suppl 1(Suppl):153-7.
Five owl monkeys, Aotus trivirgatus, were immunized against falciparum malaria by the intravenous injection of gamma-irradiated parasitized blood from donor monkeys with high parasitaemias. Each animal received approximately 1 x 10(8) parasitized erythrocytes per immunizing dose. Three monkeys received 4 doses and 2 monkeys received a single dose. After challenge, 3 control monkeys that received saline and the 2 monkeys that received a single dose of parasitized blood died with fulminant parasitaemias. The 3 monkeys that received 4 immunizing doses had either no parasitaemia at all (one monkey) or low-grade (<2.5%) parasitaemias (two monkeys); one of the latter monkeys died after the malaria had run a prolonged course. No striking haematological abnormalities were noted in animals as a result of the immunization procedure. These results confirm that immunity to Plasmodium falciparum can be induced in owl monkeys by inoculation with parasitized erythrocytes that have not undergone culture and without the use of adjuvant.
五只夜猴（Aotus trivirgatus）通过静脉注射来自供体猴的经γ射线辐照的高疟原虫血症寄生血液，对恶性疟原虫进行免疫。每只动物每次免疫剂量接受约1×10⁸个寄生红细胞。三只猴子接受4剂，两只猴子接受单剂。攻击后，接受生理盐水的3只对照猴和接受单剂寄生血液的2只猴子死于暴发性疟原虫血症。接受4剂免疫的3只猴子要么完全没有疟原虫血症（1只猴子），要么疟原虫血症程度较低（<2.5%）（2只猴子）；后一只猴子中的一只在疟疾病程延长后死亡。免疫程序未导致动物出现明显的血液学异常。这些结果证实，通过接种未经培养且不使用佐剂的寄生红细胞，可以在夜猴中诱导对恶性疟原虫的免疫力。
