Siddiqui W A, Taylor D W, Kan S C, Kramer K, Richmond-Crum S M, Kotani S, Shiba T, Kasumoto S
Bull World Health Organ. 1979;57 Suppl 1(Suppl):199-203.
The replacement of Freund's adjuvant by a possible safe adjuvant for effective immunization of owl monkeys (Aotus trivirgatus griseimembra) against a human malaria parasite, Plasmodium falciparum, has been investigated. Experiments involved the use of two synthetic adjuvants: MDP (N-acetylmuramyl-L-alanyl-D-isoglutamine) and stearoyl-MDP (6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine). In both cases, P. falciparum merozoites obtained through short-term in vitro cultivation were used as antigen. MPD was used as adjuvant in 5 owl monkeys; 2 control monkeys died and of the 3 experimental monkeys only 1 survived. In contrast, in another experiment where stearoyl-MDP was used as adjuvant, there was 100% protection of 4 immunized monkeys against a challenge with the homologous strain of P. falciparum. The results of the second experiment are encouraging for the development of an effective and safe vaccine for human malaria.
人们已经研究了用一种可能安全的佐剂替代弗氏佐剂，以便有效地免疫夜猴（Aotus trivirgatus griseimembra）使其抵抗人类疟原虫——恶性疟原虫。实验使用了两种合成佐剂：MDP（N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺）和硬脂酰-MDP（6-O-硬脂酰-N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺）。在这两种情况下，通过短期体外培养获得的恶性疟原虫裂殖子用作抗原。MPD用作5只夜猴的佐剂；2只对照猴死亡，3只实验猴中仅1只存活。相比之下，在另一项使用硬脂酰-MDP作为佐剂的实验中，4只免疫猴在受到同源恶性疟原虫株攻击时得到了100%的保护。第二项实验的结果对于开发一种有效且安全的人类疟疾疫苗而言是令人鼓舞的。
