Weisse I, Ganz H, Knappen F
Naunyn Schmiedebergs Arch Pharmacol. 1975;291(4):385-94. doi: 10.1007/BF00501796.
A method of measuring pupillary diameter in the dog using a Kowa RC-2 fundus camera, to which an eyepiece graticule had been fitted, is described. Pupil diameter was measured either directly by reading off from the calibrated graticule or from photographs. The pupillary diameters of 105 (53 male, 52 female) untreated English beagles aged between 8 and 32 months were measured under light intensities of 150-200 lux and 1000-1400 lux. Mydriatic and miotic pupillary diameters were measured in 62 male and 80 female dogs. Time-effect curves were plotted for two groups of 5 beagles receiving 75 mug/kg or 175 mug/kg atropine and 100 mug/kg or 300 mug/kg propantheline intravenously. The dose-effect curve for propantheline was found to be linear. A dose of 109 (97-121) mug/kg propantheline increased pupillary diameter by 3 mm, 20 min after administration. The time- and dose-effect curves, the significant sex differences and age dependency seen in this study indicate both a high degree of accuracy and the suitability of both of these methods for use in pharmacological and toxicological studies.
本文描述了一种使用配有目镜测微尺的柯瓦RC - 2眼底照相机测量犬瞳孔直径的方法。瞳孔直径可直接从校准的测微尺上读取，也可通过照片测量。在150 - 200勒克斯和1000 - 1400勒克斯的光照强度下，测量了105只（53只雄性，52只雌性）年龄在8至32个月之间未经治疗的英国比格犬的瞳孔直径。在62只雄性和80只雌性犬中测量了散瞳和缩瞳后的瞳孔直径。对两组各5只比格犬静脉注射75微克/千克或175微克/千克阿托品以及100微克/千克或300微克/千克丙胺太林，并绘制时间效应曲线。发现丙胺太林的剂量效应曲线呈线性。静脉注射109（97 - 121）微克/千克丙胺太林后20分钟，瞳孔直径增加3毫米。本研究中观察到的时间和剂量效应曲线、显著的性别差异和年龄依赖性表明，这两种方法具有高度准确性，适用于药理学和毒理学研究。
