Vehmeyer Stephan B W, Slooff Arnoud R M, Bloem Rolf M, Petit Pieter L C
Netherlands Bone Bank Foundation, Leiden.
Acta Orthop Scand. 2002 Apr;73(2):165-9. doi: 10.1080/000164702753671740.
We analyzed the bacterial contamination rate of femoral head allografts from living donors and determined the true bacterial load with cultures from the grafts in their entirety in a specially prepared medium. During 4 years we took swab cultures from 2,679 grafts of which 2,414 (90%) were negative. In a period of 12 months, grafts rejected for reasons other than infectious disease were cultured in their entirety to determine the true bacterial load. Of the 106 grafts included, 15 were contaminated. Microorganisms were isolated from 10 of the 91 initially swab culture-negative grafts (9%) and from only 5 of the 15 swab culture-positive grafts, most of which were normal skin contaminants. Swab culture negative grafts apparently may still be contaminated. It seems wise to subject all femoral head allografts from living donors to antibacterial processing.
我们分析了来自活体供体的股骨头同种异体移植物的细菌污染率，并通过在特制培养基中对移植物进行整体培养来确定真实的细菌载量。在4年期间，我们对2679个移植物进行了拭子培养，其中2414个（90%）为阴性。在12个月的时间里，对因非传染病原因被拒收的移植物进行了整体培养，以确定真实的细菌载量。在纳入的106个移植物中，有15个被污染。在最初拭子培养为阴性的91个移植物中，有10个（9%）分离出微生物，而在15个拭子培养为阳性的移植物中，只有5个分离出微生物，其中大多数是正常的皮肤污染物。拭子培养阴性的移植物显然仍可能被污染。对来自活体供体的所有股骨头同种异体移植物进行抗菌处理似乎是明智的。
