Berticat Claire, Rousset François, Raymond Michel, Berthomieu Arnaud, Weill Mylène
Institut des Sciences de l'Evolution (UMR 5554), Laboratoire Génétique et Environnement, CC 065, Université de Montpellier II, 34095, Montpellier cedex 05, France.
Proc Biol Sci. 2002 Jul 7;269(1498):1413-6. doi: 10.1098/rspb.2002.2022.
Wolbachia symbionts are responsible for various alterations in host reproduction. The effects of the host genome on endosymbiont levels have often been suggested, but rarely described. Here, we show that Wolbachia density is strongly modified by the presence of insecticide-resistant genes in the common house mosquito, Culex pipiens. The Wolbachia density was estimated using a real-time quantitative PCR assay. Strains harbouring different genes conferring resistance were more infected than a susceptible strain with the same genetic background. We show that this interaction also operates in natural populations. We propose that mosquitoes may control Wolbachia density less efficiently when they carry an insecticide-resistant gene, i.e. when they suffer from a physiological resistance cost.
沃尔巴克氏体共生菌会引起宿主繁殖方面的多种变化。宿主基因组对胞内共生菌水平的影响常被提及，但鲜有描述。在此，我们表明，在常见家蚊库蚊中，抗杀虫剂基因的存在会强烈改变沃尔巴克氏体的密度。使用实时定量PCR检测法估算沃尔巴克氏体的密度。携带不同抗性基因的菌株比具有相同遗传背景的敏感菌株感染程度更高。我们表明这种相互作用在自然种群中也存在。我们提出，当蚊子携带抗杀虫剂基因时，即当它们承受生理抗性代价时，可能对沃尔巴克氏体密度的控制效率较低。
