Kos-Kudla Beata, Ostrowska Zofia, Marek Bogdan, Kajdaniuk Dariusz, Ciesielska-Kopacz Nelly, Kudla Marek, Mazur Bogdan, Glogowska-Szelag Joanna, Nasiek Maja
Department of Pathophysiology and Endocrinology, Silesian Medical University, Zabrze, Poland.
Neuro Endocrinol Lett. 2002 Jun;23(3):243-8.
The aim of the study was to check if in postmenopausal women such a disease as asthma and use of hormone replacement therapy (HRT) influence daily melatonin (MEL) secretion.
Studies were performed in 55 asthmatics (treated and not treated with glucocorticosteroids) and 20 healthy postmenopausal women (aged 48-60) before HRT and after 6 months of transdermal 17beta-estradiol and medroxyprogesterone acetate treatment (cyclical method). During the circadian study blood samples for the measurement of MEL were collected every 3 hours during the day. MEL concentrations were assessed with the use of RIA methods. Statistical analysis of the circadian rhythm was performed with the use of cosinor test according to Halberg et al.
Existence of daily rhythm of MEL secretion was shown in all studied groups, before as well as after HRT. A significant decrease of mesor and amplitude's rhythm was observed in the group of women with asthma treated with glucocorticosteroids (GC) before and after HRT use in relation to women not treated with GC and the control group. Secretion of MEL in these patients was lower at nocturnal hours. A significant decrease of mean daily MEL secretion in relation to values before HRT use was shown in all groups.
Asthmatic postmenopausal women treated with GC show lowered circadian secretion of melatonin as a consequence of lowering its secretion at nocturnal hours. Hormonal replacement therapy causes a decrease of daily melatonin secretion in healthy as well as asthmatic women, not disturbing circadian rhythm of this hormone's secretion.
本研究旨在检验绝经后女性中哮喘这种疾病以及激素替代疗法（HRT）的使用是否会影响每日褪黑素（MEL）的分泌。
对55名哮喘患者（接受和未接受糖皮质激素治疗）以及20名健康绝经后女性（年龄48 - 60岁）进行研究，分别在HRT前以及采用经皮17β - 雌二醇和醋酸甲羟孕酮治疗6个月后（周期疗法）进行。在昼夜节律研究期间，白天每3小时采集一次用于测量MEL的血样。使用放射免疫分析方法评估MEL浓度。根据哈尔伯格等人的方法，采用余弦分析对昼夜节律进行统计分析。
所有研究组在HRT前后均显示出MEL分泌的昼夜节律。与未接受糖皮质激素治疗的女性和对照组相比，接受糖皮质激素（GC）治疗的哮喘女性组在HRT前后，其平均变化节律和振幅节律均显著降低。这些患者夜间的MEL分泌较低。所有组相对于HRT使用前的值，平均每日MEL分泌均显著降低。
接受GC治疗的绝经后哮喘女性由于夜间分泌减少，导致褪黑素的昼夜分泌降低。激素替代疗法会使健康女性和哮喘女性的每日褪黑素分泌减少，但不会干扰该激素分泌的昼夜节律。
