fixA和fixB基因对于大肠杆菌中的厌氧肉碱还原是必需的。
The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli.
作者信息
Walt Angelique, Kahn Michael L
机构信息
Institute of Biological Chemistry and School of Molecular Biosciences, Washington State University, Pullman 99164-6340, USA.
出版信息
J Bacteriol. 2002 Jul;184(14):4044-7. doi: 10.1128/JB.184.14.4044-4047.2002.
Abstract
In Escherichia coli, the use of carnitine as a terminal electron acceptor depends on a functional caiTABCDE operon. It had been suggested that the adjacent but divergent fixABCX operon is also required for carnitine metabolism, perhaps to provide electrons for carnitine reduction. We have constructed E. coli fixA and fixB mutants and find that they are unable to reduce carnitine to gamma-butyrobetaine under anaerobic conditions.
摘要
在大肠杆菌中，将肉碱用作末端电子受体取决于功能性的caiTABCDE操纵子。有人提出，相邻但方向相反的fixABCX操纵子对于肉碱代谢也是必需的，可能是为肉碱还原提供电子。我们构建了大肠杆菌fixA和fixB突变体，发现它们在厌氧条件下无法将肉碱还原为γ-丁酸甜菜碱。
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