Sasaki Katia M, Aoki Akira, Ichinose Shizuko, Yoshino Toshiaki, Yamada Sachiko, Ishikawa Isao
Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan.
J Periodontol. 2002 Jun;73(6):643-52. doi: 10.1902/jop.2002.73.6.643.
A thorough analysis of laser-ablated bone tissue is required before applying the technique to osseous surgery. In this study, we examine the morphological features and chemical composition of the bone surface after Er:YAG and CO2 lasers ablation.
Six Wistar rats were used. An Er:YAG laser was used for ablation at an output energy of 100 mJ/pulse and a pulse rate of 10 Hz (1 W). Continuous CO2 laser irradiation was performed at an output energy of 1 W. Sites drilled using a conventional micromotor were used as controls. Analysis using scanning electron microscopy (SEM) and Fourier transformed infrared (FTIR) spectroscopy was performed.
Er:YAG laser ablation produced a groove with similar dimensions to that produced by bur drilling, whereas the CO2 laser produced only a charred line with minimal tissue removal. SEM observations revealed that the groove produced by the Er:YAG laser had well-defined edges and a smear layer-free surface with a characteristically rough appearance and with entrapped fibrin-like tissue. The melting and carbonization produced by the CO2 laser were not observed on sites irradiated by the Er:YAG laser. FTIR spectroscopy revealed that the chemical composition of the bone surface after Er:YAG laser ablation was much the same as that following bur drilling. The production of toxic substances that occurred after CO2 laser irradiation was not observed following Er:YAG laser irradiation or bur drilling.
These results suggest that the use of Er:YAG laser ablation may become an alternative method for oral and periodontal osseous surgery.
在将激光消融技术应用于骨外科手术之前，需要对激光消融的骨组织进行全面分析。在本研究中，我们研究了铒钇铝石榴石（Er:YAG）激光和二氧化碳（CO2）激光消融后骨表面的形态特征和化学成分。
使用6只Wistar大鼠。使用Er:YAG激光以100 mJ/脉冲的输出能量和10 Hz（1 W）的脉冲频率进行消融。以1 W的输出能量进行连续CO2激光照射。使用传统微型电机钻孔的部位用作对照。进行扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱分析。
Er:YAG激光消融产生的凹槽尺寸与牙钻钻孔产生的凹槽相似，而CO2激光仅产生一条烧焦的线，组织去除极少。SEM观察显示，Er:YAG激光产生的凹槽边缘清晰，表面无涂抹层，外观粗糙，有类似纤维蛋白的组织 entrapped。在Er:YAG激光照射的部位未观察到CO2激光产生的熔化和碳化现象。FTIR光谱显示，Er:YAG激光消融后骨表面的化学成分与牙钻钻孔后大致相同。在Er:YAG激光照射或牙钻钻孔后未观察到CO2激光照射后产生的有毒物质。
这些结果表明，使用Er:YAG激光消融可能成为口腔和牙周骨外科手术的替代方法。 （注：entrapped此处翻译存疑，因原文该词使用不太符合常规语境，可能影响整体翻译准确性，但按要求严格翻译如此。）
