Hamilton Marilyn S, Abel David M, Ballam Yolanda J, Otto Mary K, Nickell Angela F, Pence Lisa M, Appleman James R, Shimasaki Craig D, Achyuthan Komandoor E
University of Missouri-Kansas City School of Medicine, Kansas City, Missouri, USA.
J Clin Microbiol. 2002 Jul;40(7):2331-4. doi: 10.1128/JCM.40.7.2331-2334.2002.
Exploiting the high sensitivity of the chemiluminescence phenomenon, an accurate and sensitive point-of-care test, called the ZstatFlu-II test (ZymeTx, Inc., Oklahoma City, Okla.), was developed to detect influenza virus infections. The ZstatFlu-II test takes 20 min and requires approximately 2 min of "hands-on" time for operational steps. The ZstatFlu-II test does not distinguish between infections with influenza virus types A and B. ZstatFlu-II test results are printed on Polaroid High-Speed Detector Film, allowing test results to be archived. A prototype version of the ZstatFlu-II test was evaluated during the 2000-to-2001 flu season with 300 nasal aspirate specimens from children at a pediatric hospital. Compared to culture, the ZstatFlu-II test had 88% sensitivity and 92% specificity. The Directigen test had a sensitivity of 75% and a specificity of 93%. The sensitivity of the ZstatFlu-II test was significantly higher than that of the Directigen test (P < 0.0574).
利用化学发光现象的高灵敏度，开发了一种名为ZstatFlu-II检测法（ZymeTx公司，俄克拉何马城，俄克拉何马州）的准确且灵敏的即时检测方法，用于检测流感病毒感染。ZstatFlu-II检测法耗时20分钟，操作步骤所需的“实际操作”时间约为2分钟。ZstatFlu-II检测法无法区分甲型和乙型流感病毒感染。ZstatFlu-II检测结果打印在宝丽来高速检测胶片上，便于存档检测结果。在2000至2001年流感季节，对一家儿童医院300份儿童鼻吸液标本进行了ZstatFlu-II检测法的原型评估。与培养法相比，ZstatFlu-II检测法的灵敏度为88%，特异性为92%。Directigen检测法的灵敏度为75%，特异性为93%。ZstatFlu-II检测法的灵敏度显著高于Directigen检测法（P < 0.0574）。
