Frost Michael, Jarboe Elke A, Orlicky David, Gianani Roberto, Thompson L Chesney, Enomoto Takayuki, Shroyer Kenneth R
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262, USA.
Am J Clin Pathol. 2002 May;117(5):738-44. doi: 10.1309/6V09-38K3-JQ40-UR50.
Survivin is an inhibitor of apoptosis protein (IAP) that is expressed in fetal development and in cancer Survivin expression in premalignant lesions remains undefined. We obtained 73 samples of cervical squamous tissue, including 31 normal, 17 low- and 15 high-grade squamous intraepithelial lesions (LSILs, HSILs), and 10 squamous cell carcinomas (SCCs)from cone biopsy and hysterectomy specimens, and stained for survivin using an immunoperoxidase method. Nuclear staining was detected in normal mucosa, LSILs, and HSILs; staining intensity was greatest in cases with morphologic evidence of human papillomavirus (HPV) infection. In situ hybridization of serial sections demonstrated colocalization of HPV DNA and survivin. Cytoplasmic staining was observed in immature squamous metaplasia and in SCCs. Survivin expression in immature metaplastic squamous mucosa may reflect a rolefor survivin in normal squamous differentiation. However, the histologic correlation between nuclear staining and HPV infection suggests involvement of survivin in HPV-mediated disruption of normal cellular maturation.
存活素是一种凋亡抑制蛋白（IAP），在胎儿发育和癌症中表达。其在前体恶性病变中的表达仍不明确。我们从锥形活检和子宫切除标本中获取了73份宫颈鳞状组织样本，包括31份正常样本、17份低级别和15份高级别鳞状上皮内病变（LSIL、HSIL）以及10份鳞状细胞癌（SCC），并采用免疫过氧化物酶法对存活素进行染色。在正常黏膜、LSIL和HSIL中均检测到核染色；在有人类乳头瘤病毒（HPV）感染形态学证据的病例中染色强度最大。连续切片的原位杂交显示HPV DNA与存活素共定位。在未成熟鳞状化生和SCC中观察到细胞质染色。存活素在未成熟化生鳞状黏膜中的表达可能反映了存活素在正常鳞状分化中的作用。然而，核染色与HPV感染之间的组织学相关性表明存活素参与了HPV介导的正常细胞成熟破坏过程。
