生存素启动子多态性与宫颈癌发生
Survivin promoter polymorphism and cervical carcinogenesis.
作者信息
Borbély A A, Murvai M, Szarka K, Kónya J, Gergely L, Hernádi Z, Veress G
机构信息
Department of Medical Microbiology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary.
出版信息
J Clin Pathol. 2007 Mar;60(3):303-6. doi: 10.1136/jcp.2006.037804. Epub 2006 May 19.
BACKGROUND
Survivin, a novel member of the inhibitor of apoptosis family, plays an important role in cell cycle regulation. A common polymorphism at the survivin gene promoter (G/C at position 31) was shown to be correlated with survivin gene expression in cancer cell lines.
AIM
To investigate whether this polymorphism could be involved in the development of human papillomavirus (HPV)-associated cervical carcinoma.
METHODS
Survivin promoter polymorphism was detected in patients with cervical cancer, in patients with equivocal cytological atypia and in a control population using polymerase chain reaction (PCR-restriction fragment length polymorphism (RFLP) and PCR-single strand conformation polymorphism analysis. HPV was typed in patients with cervical cancer and cytological atypia using PCR-RFLP.
RESULTS
No statistically significant differences were found in the genotype distributions of the survivin promoter variants among our study groups.
CONCLUSIONS
The survivin promoter polymorphism at position 31 may not represent an increased risk for the development of cervical cancer, at least in the population studied here.
背景
生存素是凋亡抑制蛋白家族的一个新成员，在细胞周期调控中起重要作用。生存素基因启动子的一个常见多态性（第31位的G/C）已被证明与癌细胞系中的生存素基因表达相关。
目的
研究这种多态性是否参与人乳头瘤病毒（HPV）相关宫颈癌的发生发展。
方法
采用聚合酶链反应（PCR-限制性片段长度多态性（RFLP）和PCR-单链构象多态性分析）检测宫颈癌患者、细胞学非典型意义不明确患者及对照人群中的生存素启动子多态性。采用PCR-RFLP对宫颈癌和细胞学非典型患者进行HPV分型。
结果
在我们的研究组中，生存素启动子变体的基因型分布没有统计学上的显著差异。
结论
至少在本研究人群中，第31位的生存素启动子多态性可能并不代表宫颈癌发生风险增加。
Zotero 插件