Hukkanen Veijo, Vuorinen Tytti
Department of Virology, University of Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland.
J Clin Virol. 2002 Jul;25 Suppl 1:S87-94. doi: 10.1016/s1386-6532(02)00038-0.
Polymerase chain reaction (PCR) is becoming the new standard for virological diagnosis of the infections of the central nervous system (CNS). Varicella-zoster virus (VZV) has been considered as the leading cause of viral meningitis or encephalitis in Finland, herpes simplex viruses (HSV) and enteroviruses being the next common causative agents.
To elucidate the roles of viruses in infections of the CNS by use of novel, sensitive time-resolved fluorescence (TRF) PCR assays.
We have utilized TRF PCR assays for diagnostics of HSV, VZV, human cytomegalovirus (CMV) and enteroviruses in infections of the CNS. When relevant, we have also applied virus culture and CSF IgM antibody determinations to elucidate the involvement of other viruses in the CNS infections. The material consisted of CSF samples from hospitals in Western Finland, submitted for diagnostic testing for CNS viral infections during the years 2000-2001. A total of 922 CSF samples were tested by PCR.
The PCR assays yielded the virological diagnosis in 72 cases whereas only 24 samples were positive by virus culture or CSF IgM antibody assays. Enteroviruses were found in 6.8%, VZV in 5.8% and HSV in 4.6% of the studied CSF samples. The virus culture and CSF antibody tests yielded 0.5-3.2% positive findings.
The PCR assays of CSF specimens are most effective for the virological diagnosis of CNS infections. In our study, HSV was the most common causative agent, whereas the diagnostic power of TRF-PCR test was highest for enteroviruses.
聚合酶链反应（PCR）正成为中枢神经系统（CNS）感染病毒学诊断的新标准。在芬兰，水痘带状疱疹病毒（VZV）被认为是病毒性脑膜炎或脑炎的主要病因，单纯疱疹病毒（HSV）和肠道病毒是其次常见的病原体。
通过使用新型、灵敏的时间分辨荧光（TRF）PCR检测方法，阐明病毒在中枢神经系统感染中的作用。
我们利用TRF PCR检测方法诊断中枢神经系统感染中的HSV、VZV、人巨细胞病毒（CMV）和肠道病毒。在相关情况下，我们还应用病毒培养和脑脊液IgM抗体检测来阐明其他病毒在中枢神经系统感染中的作用。材料包括2000 - 2001年期间从芬兰西部医院提交用于中枢神经系统病毒感染诊断检测的脑脊液样本。总共922份脑脊液样本通过PCR进行检测。
PCR检测在72例中做出了病毒学诊断，而通过病毒培养或脑脊液IgM抗体检测仅有24份样本呈阳性。在所研究的脑脊液样本中，肠道病毒占6.8%，VZV占5.8%，HSV占4.6%。病毒培养和脑脊液抗体检测的阳性率为0.5 - 3.2%。
脑脊液标本的PCR检测对中枢神经系统感染的病毒学诊断最为有效。在我们的研究中，HSV是最常见的病原体，而TRF - PCR检测对肠道病毒的诊断能力最高。
