[不同实验条件下大鼠肝脏转运核糖核酸的甲基化作用]
[Methylation of rat liver tRNA under different experimental conditions].
作者信息
Kucherenko M E, Havrylei V I
出版信息
Ukr Biokhim Zh. 1975 Mar-Apr;47(2):205-8.
The primary biochemical and conformational changes in tRNA of the albino rat liver were studied under conditions of X-ray irradiation and under the effect of prozerine and nicotinic acid. Under the effect of these factors the rate of the liver tRNA methylation increases by 45, 103 and 112%, respectively. The melting point of the tRNA control preparations is 61 degrees C, under the the effect of irradiation--57 degrees C, under the prozerine and nicotinic acid effect--52 degrees C. Hyperchromia of the tRNA control and experimental preparations is equal to 28, 30 and 34%, respectively. No changes were found in sedimentation coefficient of the tRNA control and experimental preparations.
在X射线照射条件下以及在毒扁豆碱和烟酸的作用下，对白化大鼠肝脏tRNA的主要生化和构象变化进行了研究。在这些因素的作用下，肝脏tRNA甲基化速率分别提高了45%、103%和112%。tRNA对照制剂的熔点为61℃，在照射作用下为57℃，在毒扁豆碱和烟酸作用下为52℃。tRNA对照和实验制剂的增色效应分别为28%、30%和34%。未发现tRNA对照和实验制剂的沉降系数有变化。
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