通过流式细胞术检测神经内分泌恶性肿瘤中的CD56+/CD45-免疫表型。
Detection of the CD56+/CD45- immunophenotype by flow cytometry in neuroendocrine malignancies.
作者信息
Bryson G J, Lear D, Williamson R, Wong R C W
机构信息
Division of Immunology, Queensland Health Pathology Services, Royal Brisbane Hospital, Herston, Queensland 4029, Australia.
出版信息
J Clin Pathol. 2002 Jul;55(7):535-7. doi: 10.1136/jcp.55.7.535.
AIMS
Antibodies against CD56 are primarily used in flow cytometric studies to detect natural killer cells. However, they may be useful in the identification of neuroendocrine malignancies, especially if the cells do not express CD45, indicating a non-leucocyte origin.
METHODS
A retrospective review was conducted on all solid tissue flow cytometric studies performed between January 1997 and September 2001, to identify all cases with a CD56+/CD45- immunophenotype.
RESULTS
Twelve neuroendocrine malignancies (five metastatic small cell carcinomas, three Merkel cell carcinomas, two metastatic undifferentiated neuroendocrine carcinomas, one metastatic pancreatic neuroendocrine carcinoma, and one neuroblastoma) were identified.
CONCLUSIONS
CD56+/CD45- neuroendocrine malignancies are only rarely detected in the flow cytometric analysis of solid tissue samples. However, the recognition of this immunophenotype is important to avoid their misclassification as natural killer cell malignancies. Furthermore, flow cytometry assists in the rapid identification of such cases, so that appropriate immunohistochemical studies can be performed to facilitate their correct diagnosis.
目的
抗CD56抗体主要用于流式细胞术研究以检测自然杀伤细胞。然而，它们可能有助于神经内分泌恶性肿瘤的鉴别，特别是当细胞不表达CD45时，提示其非白细胞起源。
方法
对1997年1月至2001年9月期间进行的所有实体组织流式细胞术研究进行回顾性分析，以识别所有具有CD56 + / CD45 -免疫表型的病例。
结果
共识别出12例神经内分泌恶性肿瘤（5例转移性小细胞癌、3例默克尔细胞癌、2例转移性未分化神经内分泌癌、1例转移性胰腺神经内分泌癌和1例神经母细胞瘤）。
结论
在实体组织样本的流式细胞术分析中，很少检测到CD56 + / CD45 -神经内分泌恶性肿瘤。然而，识别这种免疫表型对于避免将其误诊为自然杀伤细胞恶性肿瘤很重要。此外，流式细胞术有助于快速识别此类病例，以便能够进行适当的免疫组织化学研究以促进其正确诊断。
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