ter Brugge P J, Jansen J A
Department of Biomaterials, College of Dental Science, University Medical Center Nijmegen, The Netherlands.
Biomaterials. 2002 Aug;23(15):3269-77. doi: 10.1016/s0142-9612(02)00085-6.
Initial interactions of rat bone marrow (RBM) cells with smooth titanium, rough titanium or calcium phosphate coated substrates were tested. Cells were seeded onto the substrates, and attachment, integrin expression and spreading and morphology were studied. We found no difference in attachment of RBM cells to the different materials. We did find differences in the percentage of attached cells within a certain time between replicate runs of the experiments. RBM cells on all materials express alpha1, alpha3, alpha5, alpha6 and beta1 subunits. Again there was a large difference in expression patterns on RBM cells in different runs. No difference was found in expression on the various materials. For alpha1, alpha5, alpha6 and beta1, no difference was found in expression between attached and unattached cells. Expression of alpha3 was similar on attached and unattached cells during early culture. At the end of culture, alpha3 expression was downregulated for attached cells and not for unattached cells. This resulted in a higher expression of alpha3 for unattached cells compared to attached cells. Cells did spread on all materials, and reached a larger cell size on smooth titanium than on the rough materials. Morphology of the cells on the materials differed. On smooth titanium, cells usually showed a compact cell body with short cellular extensions. On the rough materials, cells often showed elongated shapes, with many thin cellular extensions. From this we conclude that the substrate surface characteristics of the materials we used do not influence attachment or integrin expression during the initial cell-material interactions. On the other hand, spreading behavior and cell morphology do depend on substrate surface characteristics.
测试了大鼠骨髓（RBM）细胞与光滑钛、粗糙钛或磷酸钙涂层底物的初始相互作用。将细胞接种到底物上，并研究细胞附着、整合素表达、铺展和形态。我们发现RBM细胞附着于不同材料上没有差异。我们确实发现在实验的重复操作中，特定时间内附着细胞的百分比存在差异。所有材料上的RBM细胞均表达α1、α3、α5、α6和β1亚基。同样，不同操作中RBM细胞的表达模式存在很大差异。在各种材料上的表达未发现差异。对于α1、α5、α6和β1，附着细胞和未附着细胞之间的表达未发现差异。在早期培养期间，附着细胞和未附着细胞上α3的表达相似。在培养结束时，附着细胞的α3表达下调，而未附着细胞则未下调。这导致未附着细胞的α3表达高于附着细胞。细胞确实在所有材料上铺展，并且在光滑钛上比在粗糙材料上达到更大的细胞大小。材料上细胞的形态不同。在光滑钛上，细胞通常显示出紧凑的细胞体和短的细胞突起。在粗糙材料上，细胞经常显示出细长的形状，有许多细小的细胞突起。由此我们得出结论，我们使用的材料的底物表面特性在初始细胞 - 材料相互作用期间不影响附着或整合素表达。另一方面，铺展行为和细胞形态确实取决于底物表面特性。
