ter Brugge P J, Torensma R, De Ruijter J E, Figdor C G, Jansen J A
Department of Biomaterials, College of Dental Science, University Medical Center Nijmegen, Nijmegen, The Netherlands.
Tissue Eng. 2002 Aug;8(4):615-26. doi: 10.1089/107632702760240535.
Biomaterials have been shown to be able to influence the growth and differentiation of osteogenic cells cultured on the surface. Although the precise mechanisms by which the materials influence osteogenic cells are unclear, it is possible that the materials manipulate the expression of integrins by the cells. We therefore studied the expression of a number of integrins by rat bone marrow (RBM) cells, after culture on culture polystyrene, on machined and grit-blasted titanium, and on calcium phosphate-coated titanium. Integrin expression was studied by FACS analysis. We found a large variation in the expression of integrins by cells in replicate experiments. After culture on polystyrene for 7 days, cells expressed alpha1, alpha2, alpha3, alpha5, alpha6, beta1, and beta3, although some of the subunits were expressed only occasionally. The cells did not express the alpha4 subunit. After culture of RBM cells for 8 days on coated and noncoated titanium substrates, cells always expressed alpha3, alpha5, alpha6, and beta1. The alpha1 and beta3 subunits were only expressed in some of the experiments. Frequently, the expression of alpha5, alpha6, and beta1 was higher on the coated than on the noncoated titanium substrates. Based on our results, we conclude that the studied materials are capable of influencing the expression of integrins by RBM cells cultured on relevant implant materials.
生物材料已被证明能够影响在其表面培养的成骨细胞的生长和分化。尽管材料影响成骨细胞的确切机制尚不清楚，但材料可能会操纵细胞中整合素的表达。因此，我们研究了大鼠骨髓（RBM）细胞在聚苯乙烯培养皿、机械加工和喷砂处理的钛以及磷酸钙涂层钛上培养后多种整合素的表达情况。通过流式细胞术分析研究整合素的表达。我们发现在重复实验中细胞整合素的表达存在很大差异。在聚苯乙烯上培养7天后，细胞表达α1、α2、α3、α5、α6、β1和β3，尽管有些亚基只是偶尔表达。细胞不表达α4亚基。RBM细胞在涂覆和未涂覆的钛基底上培养8天后，细胞总是表达α3、α5、α6和β1。α1和β3亚基仅在部分实验中表达。通常，α5、α6和β1在涂覆钛基底上的表达高于未涂覆的钛基底。基于我们的结果，我们得出结论，所研究的材料能够影响在相关植入材料上培养的RBM细胞中整合素的表达。
