Kumazawa Ryuichiro, Watari Fumio, Takashi Noriyuki, Tanimura Yukihiro, Uo Motohiro, Totsuka Yasunori
Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Biomaterials. 2002 Sep;23(17):3757-64. doi: 10.1016/s0142-9612(02)00115-1.
We compared the cytotoxicity of soluble and particulate titanium (Ti), vanadium (V) and nickel (Ni) by biochemical functional analysis and by microscopic morphology with micro-area elemental analysis in vitro using human neutrophils as probes and in vivo in animals. The biochemical analyses of LDH, superoxide anion, TNF-alpha and scanning electron microscopy (SEM) showed that Ni in solution destroys the cell membrane of neutrophils, whereas Ti and V in solution stimulate neutrophils and increase the quantity of released superoxide anions. Fine Ti particles (1-3 microm), which smaller than neutrophils (about 5 microm), were phagocytized by the cells and the results were similar in vivo. These results showed that the cytotoxic effect of Ti particles is size dependent, and that they must be smaller than that of cells. The present study demonstrated that the biochemical functional tests are useful for evaluating the biocompatibility of materials.
我们通过生化功能分析以及使用人类中性粒细胞作为探针进行体外微观形态与微区元素分析，并在动物体内进行研究，比较了可溶性和颗粒状钛（Ti）、钒（V）和镍（Ni）的细胞毒性。对乳酸脱氢酶（LDH）、超氧阴离子、肿瘤坏死因子-α的生化分析以及扫描电子显微镜（SEM）显示，溶液中的镍会破坏中性粒细胞的细胞膜，而溶液中的钛和钒会刺激中性粒细胞并增加释放的超氧阴离子数量。细小的钛颗粒（1-3微米），比中性粒细胞（约5微米）小，被细胞吞噬，体内结果相似。这些结果表明钛颗粒的细胞毒性作用取决于颗粒大小，且它们必须小于细胞大小。本研究表明生化功能测试对于评估材料的生物相容性很有用。
