Department of Orthopedics, Peking University Third Hospital, Beijing 100191, PR China; Beijing Key Laboratory of Spinal Diseases, Beijing 100191, PR China.
Department of Orthopedic Surgery, West China Hospital, Sichuan University, No. 37 GuoXue Road, Chengdu, PR China.
Colloids Surf B Biointerfaces. 2018 Sep 1;169:366-374. doi: 10.1016/j.colsurfb.2018.05.044. Epub 2018 May 19.
MAO-treated porous Ti6Al4V holds enormous potential for use in orthopedic implants due to their excellent biocompatibility and favourable mechanical strength. However, the effects on the V ion accumulation and release following the MAO-treated Ti6Al4V remain undetermined. The aim of the present study was to assess the effects of Vanadium on biocompatibility. In this study, the surface features and chemical compositions were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectroscopy (XPS). The ion release of Ti, Al and V was quantitatively measured by inductively-coupled plasma mass spectroscopy (ICP-MS) after immersion in Hanks' solution. To probe the mechanism of V release, the corrosion resistance of porous Ti6Al4V before and after the MAO process was evaluated by electrochemical tests. Thereafter, the effects on the biocompatibility were tested in vitro by cell culture assays and then in vivo by subcutaneous embedment. Finally, the bone tissue response and in vivo release profile of V ions were characterized by intra-osseous implantation. Therefore, this study suggests that the effect of V released from MAO-treated porous Ti6Al4V on biocompatibility and application safety is small and preventable.
经 MAO 处理的多孔 Ti6Al4V 由于其出色的生物相容性和良好的机械强度，在骨科植入物中有巨大的应用潜力。然而，MAO 处理后的 Ti6Al4V 中 V 离子的积累和释放的影响仍不确定。本研究旨在评估钒对生物相容性的影响。在这项研究中，通过扫描电子显微镜 (SEM)、能谱仪 (EDS) 和 X 射线光电子能谱 (XPS) 对表面特征和化学成分进行了表征。通过在 Hanks 溶液中浸泡，通过电感耦合等离子体质谱 (ICP-MS) 定量测量了 Ti、Al 和 V 的离子释放。为了探究 V 释放的机制，通过电化学测试评估了 MAO 处理前后多孔 Ti6Al4V 的耐腐蚀性。随后，通过体外细胞培养实验和体内皮下埋植实验测试了其对生物相容性的影响。最后，通过骨内植入实验对 V 离子的组织反应和体内释放情况进行了表征。因此，本研究表明，MAO 处理的多孔 Ti6Al4V 中释放的 V 对生物相容性和应用安全性的影响是较小的，且是可预防的。
