通过细胞质多聚腺苷酸化和多聚（A）结合蛋白溶解maskin-eIF4E复合物可控制细胞周期蛋白B1 mRNA的翻译和卵母细胞成熟。
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation.
作者信息
Cao Quiping, Richter Joel D
机构信息
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
出版信息
EMBO J. 2002 Jul 15;21(14):3852-62. doi: 10.1093/emboj/cdf353.
Abstract
Cytoplasmic polyadenylation stimulates the translation of several dormant mRNAs during oocyte maturation in Xenopus. Polyadenylation is regulated by the cytoplasmic polyadenylation element (CPE), a cis-acting element in the 3'-untranslated region of responding mRNAs, and its associated factor CPEB. CPEB also binds maskin, a protein that in turn interacts with eIF4E, the cap-binding factor. Here, we report that based on antibody and mRNA reporter injection assays, maskin prevents oocyte maturation and the translation of the CPE-containing cyclin B1 mRNA by blocking the association of eIF4G with eIF4E. Dissociation of the maskin-eIF4E complex is essential for cyclin B1 mRNA translational activation, and requires not only cytoplasmic polyadenylation, but also the poly(A)-binding protein. These results suggest a molecular mechanism by which CPE- containing mRNA is activated in early development.
摘要
在非洲爪蟾的卵母细胞成熟过程中，细胞质聚腺苷酸化会刺激几种休眠mRNA的翻译。聚腺苷酸化由细胞质聚腺苷酸化元件（CPE）调控，CPE是响应mRNA的3'非翻译区中的顺式作用元件，以及其相关因子CPEB。CPEB还与Maskin结合，Maskin是一种依次与帽结合因子eIF4E相互作用的蛋白质。在这里，我们报告基于抗体和mRNA报告基因注射试验，Maskin通过阻断eIF4G与eIF4E的结合来阻止卵母细胞成熟和含CPE的细胞周期蛋白B1 mRNA的翻译。Maskin-eIF4E复合物的解离对于细胞周期蛋白B1 mRNA的翻译激活至关重要，不仅需要细胞质聚腺苷酸化，还需要聚（A）结合蛋白。这些结果提示了一种在早期发育中激活含CPE的mRNA的分子机制。
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