Gebre-Yohannes A, Drasar B S
National Research Institute of Health, Addis Ababa, Ethiopia.
Epidemiol Infect. 1991 Oct;107(2):321-34. doi: 10.1017/s0950268800048962.
A total of 123 drug-resistant and drug-sensitive Shigella flexneri types 1-6, and their Escherichia coli K12 transconjugants were used for plasmid profile analysis by agarose gel electrophoresis. Resistance factors (R-factors) were further characterized by incompatibility testing. The overall distribution of small plasmids in S. flexneri showed that a cryptic plasmid of about 4.6 Kb was found in all serotypes, and a plasmid of about 4.2 Kb was found in serotypes 1-4. Shigella flexneri types 2, 4 and 6 showed a 6.5 Kb plasmid which correlated with SSu-resistance. All S. flexneri serotypes harboured large plasmids of about 217 Kb. Plasmid profile analysis of S. flexneri in Ethiopia showed a high degree of uniformity within individual serotypes. However, there was a limited variability which, at times, could be useful for epidemiological investigation. Shigella flexneri serotypes 1-6 harboured resistance plasmids with diverse molecular weights but mostly belonging to incompatibility groups N and X.
总共123株1 - 6型耐药和敏感的福氏志贺菌及其大肠杆菌K12转导接合子通过琼脂糖凝胶电泳进行质粒图谱分析。通过不相容性测试进一步鉴定耐药因子（R因子）。福氏志贺菌中小质粒的总体分布表明，在所有血清型中均发现了约4.6 Kb的隐蔽质粒，在血清型1 - 4中发现了约4.2 Kb的质粒。福氏志贺菌2型、4型和6型显示出与链霉素 - 磺胺甲恶唑耐药相关的6.5 Kb质粒。所有福氏志贺菌血清型都含有约217 Kb的大质粒。埃塞俄比亚福氏志贺菌的质粒图谱分析显示各血清型内部具有高度一致性。然而，存在有限的变异性，有时这对流行病学调查可能有用。福氏志贺菌1 - 6型携带分子量各异但大多属于不相容群N和X的耐药质粒。
