Kato Hideaki, Ruzibakiev Ruslan, Yuldasheva Nodira, Hegay Tatyana, Kurbanov Fuat, Achundjanov Bakhodir, Tuichiev Lazis, Usuda Sadakazu, Ueda Ryuzo, Mizokami Masashi
Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Japan.
J Med Virol. 2002 Aug;67(4):477-83. doi: 10.1002/jmv.10126.
Hepatitis B virus (HBV) has been classified into seven genotypes, designated A-G. The HBV genotype has a characteristic geographical distribution. The Republic of Uzbekistan is located in the heart of Asia and has been considered to be a region with high endemicity of hepatitis viruses. However, the present distribution of hepatitis virus infection in this region is unknown. The aim of this study was to investigate the distribution of HBV genotypes and to elucidate the validity of two genotyping systems in Uzbekistan. Fifty-four patients with hepatitis B surface antigen were investigated. HBV genotypes were determined by two methods: one based on restriction fragment length polymorphism (RFLP) targeting to S region, and another on enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies to pre-S2 region. Seven (13%) and 47 (87%) of the 54 subjects were classified into genotypes A and D, respectively. Dual infection of two viral populations of the same genotype was observed in one subject. No significant difference of ALT level (203.3 +/- 244.7 vs. 190.6 +/- 39.5) and HBeAg (42.9% vs. 42.6%) were found between genotypes A and D. In this study, the validity of the genotyping systems in this region was confirmed.
乙型肝炎病毒（HBV）已被分为七种基因型，分别命名为A - G。HBV基因型具有特征性的地理分布。乌兹别克斯坦共和国位于亚洲中心，被认为是肝炎病毒高流行地区。然而，该地区目前的肝炎病毒感染分布情况尚不清楚。本研究的目的是调查HBV基因型的分布，并阐明乌兹别克斯坦两种基因分型系统的有效性。对54例乙型肝炎表面抗原阳性患者进行了调查。采用两种方法确定HBV基因型：一种基于针对S区的限制性片段长度多态性（RFLP），另一种基于酶联免疫吸附测定（ELISA），使用针对前S2区的单克隆抗体。54名受试者中，7例（13%）和47例（87%）分别被归类为A基因型和D基因型。在一名受试者中观察到同一基因型的两个病毒群体的双重感染。A基因型和D基因型之间在ALT水平（203.3±244.7对190.6±39.5）和HBeAg（42.9%对42.6%）方面未发现显著差异。在本研究中，证实了该地区基因分型系统的有效性。
