Wigger-Alberti Walter, Spoo Julia, Schliemann-Willers Sibylle, Klotz Andreas, Elsner Peter
Department of Dermatology, Friedrich-Schiller-University, Jena, Germany.
Acta Derm Venereol. 2002;82(2):94-7. doi: 10.1080/00015550252948103.
The effect of a protective cream was tested in a new tandem repeated irritation test with tandem application of 0.5% sodium lauryl sulphate (SLS) and undiluted toluene. The irritants were applied twice daily for 30 min to the ventral forearms of 20 volunteers. Irritant cutaneous reactions were quantified by a visual score, transepidermal water loss, chromametry and skin capacitance. Concurrent application of SLS/toluene induced stronger reactions than those caused by twice daily application of each irritant on its own. A protective effect of the protective cream was obtained against all treatment combinations and was significant for SLS/SLS (p < or = 0.01) and SLS/ toluene (p < or = 0.05). Our results indicate that the tandem repetitive irritation test has great potential in the evaluation of skin care products to prevent irritant contact dermatitis.
在一项新的串联重复刺激试验中，通过串联使用0.5%的十二烷基硫酸钠（SLS）和未稀释的甲苯，对一种防护霜的效果进行了测试。刺激物每天两次涂抹于20名志愿者的前臂腹侧，每次30分钟。通过视觉评分、经表皮水分流失、色度测定和皮肤电容对刺激性皮肤反应进行量化。与单独每日两次使用每种刺激物相比，同时使用SLS/甲苯会引起更强的反应。该防护霜对所有处理组合均有防护作用，对SLS/SLS（p≤0.01）和SLS/甲苯（p≤0.05）具有显著意义。我们的结果表明，串联重复刺激试验在评估预防刺激性接触性皮炎的护肤品方面具有很大潜力。
