黄芪对不同癌细胞系生长的影响。
Effects of astragali radix on the growth of different cancer cell lines.
作者信息
Lin Jiang, Dong Hui-Fang, Oppenheim J J, Howard O M
机构信息
Deportment of Gastroenterology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China.
出版信息
World J Gastroenterol. 2003 Apr;9(4):670-3. doi: 10.3748/wjg.v9.i4.670.
AIM
To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell line.
METHODS
To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method.
RESULTS
The inhibition rates of AR on the cell respiration of AGS, KATOIII, HT29, MDA231, MEL7 and MEL14 were 68.25 %, 62.36 %, 22.8 %, 27.69 %, 2.85 % and 5.14 % respectively at the concentration of 100 ug/ml; it inhibited AGS DNA synthesis by 87.33 % at the concentration of 50 ug/ml. The inhibitory effect on AGS was time-and dose-dependent. AR did not induce apoptosis in AGS cells.
CONCLUSION
AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.
目的
研究中药黄芪对不同癌细胞系生长的抑制作用。
方法
采用台盼蓝排斥法、MTS法及氚胸腺嘧啶核苷掺入法观察黄芪对肿瘤细胞增殖的体外作用。采用DNA梯状法检测细胞凋亡。
结果
在100μg/ml浓度下，黄芪对AGS、KATOIII、HT29、MDA231、MEL7和MEL14细胞呼吸的抑制率分别为68.25%、62.36%、22.8%、27.69%、2.85%和5.14%；在50μg/ml浓度下，黄芪对AGS细胞DNA合成的抑制率为87.33%。黄芪对AGS细胞的抑制作用具有时间和剂量依赖性。黄芪未诱导AGS细胞凋亡。
结论
黄芪在体外可特异性抑制胃癌细胞生长，其机制主要是细胞生长抑制，而非细胞毒性或诱导凋亡。
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