来自沙门氏菌的酪氨酸可抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的特性
Properties of tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthase from Salmonella.
作者信息
Hu C Y, Sprinson D B
出版信息
J Bacteriol. 1977 Jan;129(1):177-83. doi: 10.1128/jb.129.1.177-183.1977.
Abstract
Tyrosine-inhibitable 3-deoxy-D-arabinoheptulosonic acid-7-phosphate (DAHP) synthase was purified to homogeneity without significant loss of sensitivity to inhibition by tyrosine from an operator-constitutive strain (tyrOc) of Salmonella. The enzyme had an apparent molecular weight of 76,000 by gel filtration and a subunit molecular weight of 40,000 by sodium dodecyl sulfate-gel electrophoresis and by reaction with dimethyl suberimidate. It had an isoelectric point of 4.68. Inhibition by L-tyrosine showed a Hill coefficient of 1.8 at pH 7.0, suggesting cooperative interaction between tyrosine-binding sites, and was competitive with phosphoenol pyruvate and noncompetitive with erythrose-4-phosphate.
摘要
酪氨酸可抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）合酶从沙门氏菌的一个操纵子组成型菌株（tyrOc）中纯化至同质，且对酪氨酸抑制的敏感性没有显著损失。通过凝胶过滤，该酶的表观分子量为76,000，通过十二烷基硫酸钠-凝胶电泳和与辛二酸二甲酯反应，其亚基分子量为40,000。其等电点为4.68。L-酪氨酸的抑制在pH 7.0时显示出1.8的希尔系数，表明酪氨酸结合位点之间存在协同相互作用，并且与磷酸烯醇丙酮酸竞争，与4-磷酸赤藓糖非竞争。
相似文献
1
Properties of tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthase from Salmonella.来自沙门氏菌的酪氨酸可抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的特性
J Bacteriol. 1977 Jan;129(1):177-83. doi: 10.1128/jb.129.1.177-183.1977.
2
Purification of the tyrosine inhibitable 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Schizosaccharomyces pombe.来自粟酒裂殖酵母的酪氨酸可抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的纯化。
Biochim Biophys Acta. 1977 Dec 8;485(2):446-51. doi: 10.1016/0005-2744(77)90180-2.
3
3-Deoxy-D-arabino-heptulosonic acid 7-phosphate synthase mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的3-脱氧-D-阿拉伯庚酮糖酸7-磷酸合酶突变体
J Bacteriol. 1975 Dec;124(3):1312-20. doi: 10.1128/jb.124.3.1312-1320.1975.
4
5
Pseudomonas aeruginosa possesses two novel regulatory isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.铜绿假单胞菌拥有两种新型的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶调节同工酶。
J Biol Chem. 1982 Nov 10;257(21):12789-94.
6
Fluorine-containing analogues of intermediates in the Shikimate pathway.莽草酸途径中间体的含氟类似物。
Biochemistry. 1976 Nov 30;15(24):5315-20. doi: 10.1021/bi00669a018.
7
Purification and properties of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (phenylalanine-inhibitable) of Saccharomyces cerevisiae.酿酒酵母3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶（苯丙氨酸抑制型）的纯化及性质
Eur J Biochem. 1989 Dec 8;186(1-2):361-6. doi: 10.1111/j.1432-1033.1989.tb15217.x.
8
Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. I. Properties and regulation of 3-deoxy-d-arabino heptulosonate 7-phosphate synthase.真养产碱杆菌H16中芳香族氨基酸的生物合成。I. 3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的性质与调控
Arch Microbiol. 1975 Apr 7;103(2):133-40. doi: 10.1007/BF00436340.
9
Regulatory isozymes of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase in the cyanobacterium Anabaena sp. strain ATCC 29151.蓝藻鱼腥藻属ATCC 29151菌株中3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的调控同工酶
J Bacteriol. 1981 Oct;148(1):361-4. doi: 10.1128/jb.148.1.361-364.1981.
10
The two 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isoenzymes from Saccharomyces cerevisiae show different kinetic modes of inhibition.来自酿酒酵母的两种3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶同工酶表现出不同的抑制动力学模式。
Arch Microbiol. 1998 Jun;169(6):517-24. doi: 10.1007/s002030050605.
引用本文的文献
1
3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthase from Carrot Root (Daucus carota) Is a Hysteretic Enzyme.胡萝卜根 3-脱氧-d-阿拉伯庚酮糖-7-磷酸合酶是一种滞后酶。
Plant Physiol. 1985 Nov;79(3):765-70. doi: 10.1104/pp.79.3.765.
2
Regulation of the shikimate pathway of carrot cells in suspension culture.悬浮培养的胡萝卜细胞中天冬氨酸途径的调控。
Plant Physiol. 1984 Jun;75(2):369-71. doi: 10.1104/pp.75.2.369.
3
Comparative allostery of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase as an indicator of taxonomic relatedness in pseudomonad genera.3-脱氧-D-阿拉伯庚酮糖酸7-磷酸合成酶的比较变构作用作为假单胞菌属分类相关性的一个指标
J Bacteriol. 1981 Feb;145(2):752-9. doi: 10.1128/jb.145.2.752-759.1981.
4
Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.鼠伤寒沙门氏菌aroF基因在大肠杆菌中的调控
J Bacteriol. 1990 May;172(5):2259-66. doi: 10.1128/jb.172.5.2259-2266.1990.
5
Linkage map of Salmonella typhimurium, edition V.鼠伤寒沙门氏菌连锁图谱，第五版
Microbiol Rev. 1978 Jun;42(2):471-519. doi: 10.1128/mr.42.2.471-519.1978.
6
Iron, an essential element for biosynthesis of aromatic compounds.铁，芳香族化合物生物合成所必需的元素。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4810-3. doi: 10.1073/pnas.75.10.4810.
本文引用的文献
1
Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside.腺苷和2,6-二氨基嘌呤核苷的酶促磷酸化作用。
J Biol Chem. 1951 Dec;193(2):481-95.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
4
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
5
THE SYNTHESIS OF 3-DEOXY-D-ARABINO-HEPTULOSONIC ACID 7-PHOSPHATE.3-脱氧-D-阿拉伯庚酮糖酸7-磷酸的合成
J Biol Chem. 1963 Oct;238:3170-5.
6
Allosteric proteins and cellular control systems.别构蛋白与细胞控制系统。
J Mol Biol. 1963 Apr;6:306-29. doi: 10.1016/s0022-2836(63)80091-1.
7
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.蛋白质的凝胶过滤行为与其在很宽范围内的分子量相关。
Biochem J. 1965 Sep;96(3):595-606. doi: 10.1042/bj0960595.
8
Chorismic acid: purification and some chemical and physical studies.分支酸：纯化及一些化学和物理研究
Biochem J. 1964 Feb;90(2):256-61. doi: 10.1042/bj0900256.
9
Phosphonomycin, a new antibiotic produced by strains of streptomyces.磷霉素，一种由链霉菌菌株产生的新型抗生素。
Science. 1969 Oct 3;166(3901):122-3. doi: 10.1126/science.166.3901.122.
10
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
Zotero 插件