Kobus Hilton J, Silenieks Edmund, Scharnberg Jordana
Forensic Science Centre, Adelaide, SA, Australia.
J Forensic Sci. 2002 Jul;47(4):819-23.
The paper describes the use of the Polilight, a light source based on a xenon arc lamp, to exploit the fluorescence properties of semen as an aid to searching fabrics for stains in sexual assault cases. The broad excitation spectrum of semen allows the fluorescence to be generated at a range of wavelengths. This permits the excitation and emission conditions to be selected that minimize interference from background fluorescence of the fabric and thereby optimizes the contrast between the fabric and the stain. A common method for the observation of fluorescence is the use of colored plastic goggles or filters. The paper shows that the detection of fluorescence from semen stains is significantly enhanced using appropriate interference filters.
本文描述了如何使用基于氙弧灯的光源Polilight，利用精液的荧光特性来协助在性侵犯案件中寻找织物上的污渍。精液的宽激发光谱使得荧光能够在一系列波长下产生。这允许选择激发和发射条件，以尽量减少织物背景荧光的干扰，从而优化织物与污渍之间的对比度。观察荧光的常用方法是使用有色塑料护目镜或滤光片。本文表明，使用适当的干涉滤光片可显著增强对精液污渍荧光的检测。
