Cross Martin L, Mortensen Rikke R, Kudsk Jane, Gill Harsharnjit S
Milk & Health Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand.
Med Microbiol Immunol. 2002 May;191(1):49-53. doi: 10.1007/s00430-002-0112-7.
Probiotic lactobacilli have been proposed as a potential oral bacteriotherapeutic means of modulating immune phenotype expression in vivo, via their ability to promote cytokine production. This study investigated the ability of a known interferon (IFN)gamma-promoting probiotic (Lactobacillus rhamnosus HNOOI) to modulate cytokine production in mice expressing an on-going Th2-type immune response. BALB/c mice were primed to ovalbumin in alum adjuvant to invoke antigen-specific Th2 cytokine-secreting cell populations. Mice that were fed Lb. rhamnosus HN001 during antigen sensitization produced higher levels of lymphocyte-derived IFNgamma, but also interleukin (IL)-4 and IL-5, in comparison to control animals. Although HN001 was additionally shown to induce pro-IFNgamma monokine (IL-12, IL-18) secretion in macrophages in vitro, its ability to invoke mixed lymphocyte cytokine production during an on-going Th2-type immune response in vivo suggests that this probiotic is a general immunostimulatory agent, in contrast to the pro-Th1/anti-Th2 immunoregulation reported for some strains of IFNgamma-promoting lactobacilli.
益生菌乳酸杆菌已被提议作为一种潜在的口服细菌治疗手段，通过其促进细胞因子产生的能力在体内调节免疫表型表达。本研究调查了一种已知的促进干扰素（IFN）γ的益生菌（鼠李糖乳杆菌HNOOI）调节表达持续Th2型免疫反应的小鼠细胞因子产生的能力。用卵清蛋白在明矾佐剂中对BALB/c小鼠进行致敏，以引发抗原特异性分泌Th2细胞因子的细胞群体。与对照动物相比，在抗原致敏期间喂食鼠李糖乳杆菌HN001的小鼠产生了更高水平的淋巴细胞衍生的IFNγ，以及白细胞介素（IL）-4和IL-5。尽管另外显示HN001在体外可诱导巨噬细胞分泌前IFNγ单因子（IL-12、IL-18），但其在体内持续的Th2型免疫反应期间引发混合淋巴细胞细胞因子产生的能力表明，与一些促进IFNγ的乳酸杆菌菌株所报道的促Th1/抗Th2免疫调节相反，这种益生菌是一种一般的免疫刺激剂。
