Laslett Dean, Canback Bjorn, Andersson Siv
Murdoch University, Perth, Western Australia, Australia.
Nucleic Acids Res. 2002 Aug 1;30(15):3449-53. doi: 10.1093/nar/gkf459.
A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes have been reported previously, BRUCE identified all with no false positives. In addition, BRUCE found 99 of the 100 tmRNAs identified previously in other bacteria, red chloroplasts and cyanelles. The output of the program reports the proposed tRNA secondary structure, the tmRNA gene sequence and the tag peptide.
开发了一个名为BRUCE的计算机程序,用于识别转移信使RNA(tmRNA)基因。该程序采用启发式算法,在编码标签肽的短序列周围搜索类似丙氨酸转运RNA(tRNA(Ala))的二级结构。在先前已报道存在tmRNA基因的57个完全测序的细菌基因组中,BRUCE全部识别出来,无一假阳性。此外,BRUCE在先前在其他细菌、红色叶绿体和蓝藻中鉴定出的100个tmRNA中发现了99个。该程序的输出报告了推测的tRNA二级结构、tmRNA基因序列和标签肽。